Structure of PDB 4h1s Chain A

Receptor sequence
>4h1sA (length=521) Species: 9606 (Homo sapiens) [Search protein sequence]
WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEP
NVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEG
LIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGY
TSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMD
KLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVP
VVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINK
WRIKLDNYQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDE
MFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKG
STLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTK
CRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVV
STYISKMKVIYPAVEGRIKFS
3D structure
PDB4h1s Crystal structure of a soluble form of human CD73 with ecto-5'-nucleotidase activity.
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D36 H38 D85 N117 H118 D121 H220 H243 N245 S350 R354 R395
Catalytic site (residue number reindexed from 1) D10 H12 D59 N91 H92 D95 H194 H217 N219 S322 R326 R367
Enzyme Commision number 3.1.3.35: thymidylate 5'-phosphatase.
3.1.3.5: 5'-nucleotidase.
3.1.3.89: 5'-deoxynucleotidase.
3.1.3.91: 7-methylguanosine nucleotidase.
3.1.3.99: IMP-specific 5'-nucleotidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A D85 N117 H220 H243 D59 N91 H194 H217
BS02 ZN A D36 H38 D85 D10 H12 D59
BS03 PO4 A H38 D85 N117 H118 H243 R354 R395 H12 D59 N91 H92 H217 R326 R367
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0002953 5'-deoxynucleotidase activity
GO:0005515 protein binding
GO:0008252 nucleotidase activity
GO:0008253 5'-nucleotidase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016788 hydrolase activity, acting on ester bonds
GO:0042802 identical protein binding
GO:0046872 metal ion binding
GO:0050340 thymidylate 5'-phosphatase activity
GO:0050483 IMP 5'-nucleotidase activity
GO:0050484 GMP 5'-nucleotidase activity
GO:0106411 XMP 5'-nucleosidase activity
Biological Process
GO:0006196 AMP catabolic process
GO:0006259 DNA metabolic process
GO:0007159 leukocyte cell-cell adhesion
GO:0009166 nucleotide catabolic process
GO:0033198 response to ATP
GO:0046032 ADP catabolic process
GO:0046034 ATP metabolic process
GO:0046086 adenosine biosynthetic process
GO:0050728 negative regulation of inflammatory response
GO:0055074 calcium ion homeostasis
GO:0140928 inhibition of non-skeletal tissue mineralization
Cellular Component
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0009897 external side of plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0070062 extracellular exosome
GO:0098552 side of membrane

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Molecular Function
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Biological Process
View graph for
Cellular Component
External links
PDB RCSB:4h1s, PDBe:4h1s, PDBj:4h1s
PDBsum4h1s
PubMed22997138
UniProtP21589|5NTD_HUMAN 5'-nucleotidase (Gene Name=NT5E)

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