Structure of PDB 4gnm Chain A

Receptor sequence
>4gnmA (length=608) Species: 10116 (Rattus norvegicus) [Search protein sequence]
QLHLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGR
LLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPI
PKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAK
IGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKP
LVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLA
KEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKV
ECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNT
IFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSR
FCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVF
VGAAMRSEGGIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHV
NWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDAL
NLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRA
LKQRISQM
3D structure
PDB4gnm The {Omega}-loop lid domain of phosphoenolpyruvate carboxykinase is essential for catalytic function.
ChainA
Resolution1.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R87 Y235 K244 H264 S286 C288 D311 R405
Catalytic site (residue number reindexed from 1) R82 Y230 K239 H259 S281 C283 D306 R400
Enzyme Commision number 2.7.11.-
4.1.1.32: phosphoenolpyruvate carboxykinase (GTP).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GTP A S286 A287 G289 K290 T291 N292 R405 W516 F517 F525 G529 F530 S281 A282 G284 K285 T286 N287 R400 W502 F503 F511 G515 F516
BS02 MN A K244 H264 D311 K239 H259 D306
BS03 OXL A R87 K244 H264 S286 D311 R405 R82 K239 H259 S281 D306 R400
Gene Ontology
Molecular Function
GO:0004611 phosphoenolpyruvate carboxykinase activity
GO:0004613 phosphoenolpyruvate carboxykinase (GTP) activity
GO:0005525 GTP binding
GO:0017076 purine nucleotide binding
Biological Process
GO:0006094 gluconeogenesis

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Molecular Function
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Biological Process
External links
PDB RCSB:4gnm, PDBe:4gnm, PDBj:4gnm
PDBsum4gnm
PubMed23127136
UniProtP07379|PCKGC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (Gene Name=Pck1)

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