Structure of PDB 4g32 Chain A

Receptor sequence

>4g32A (length=630) Species: 287 (Pseudomonas aeruginosa)

IPPSLPQYDNLAGKLARRAVLTSKKLVYVWTENFANVKGVPMARSVPLGE
LPNVDWLLKTAGVIVELIVNFVASLPASAAAQFERIAAGLSGDLEAARQV
HEALLEEAKNDPAAAGSLLLRFTELQTRVIALLTRVGLLVDDILKSASNL
VDGLNRFRAVFGTLRLPEVADSFRDDEAFAYWRVAGPNPLLIRRVDALPA
NFPLGEEQFRRVMGADDSLLEAAASRRLYLLDYAELGKLAPSGAVDKLLT
GTGFAYAPIALFALGKDRAGLLPVAIQCGQDPATHPMFVRPAESESDLYW
GWQMAKTVVQVAEENYHEMFVHLAQTHLVSEAFCLATQRTLAPSHPLHVL
LAPHFEGTLFINEGAARILLPSAGFIDVMFAAPIQDTQATAGGNRLGFDF
YRGMLPESLKARNVDDPAALPDYPYRDDGLLVWNAIRQWAADYVAVYYAS
DGDVTADVELAAWVGEVIGSGKVAGFRPITGRSQLVEVLTMVIFTASAQH
AAVNFPQPSMMTYAPAICAMSAAPAPDSPSGKSEADWLKMMPPTLVALEK
VNIYHLLGSVYHGRLGDYRQTGFPYAPVFSDRRVTASGGPLERFQARLKE
VEATIRTRNQARRKPYEYLLPSRIPASTNI

3D structure

• PDB: 4g32 Structure and interaction with phospholipids of a prokaryotic lipoxygenase from Pseudomonas aeruginosa.
• Chain: A
• Resolution: 1.75 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H377 H382 H555 N559 I685
• Catalytic site (residue number reindexed from 1): H322 H327 H500 N504 I630
• Enzyme Commision number: 1.13.11.12: linoleate 13S-lipoxygenase.
  1.13.11.58: linoleate 9S-lipoxygenase.
  1.13.11.77: oleate 10S-lipoxygenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE2	A	H377 H382 H555 N559 I685	H322 H327 H500 N504 I630
BS02	ZPE	A	L182 G186 D190 E373 H382 F415 A420 I423 L424	L133 G137 D141 E318 H327 F360 A365 I368 L369

Gene Ontology

Molecular Function

• GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
• GO:0046872 metal ion binding

Biological Process

• GO:0034440 lipid oxidation

External links

• PDB: RCSB:4g32, PDBe:4g32, PDBj:4g32
• PDBsum: 4g32
• PubMed: 23985801
• UniProt: Q8RNT4|LOX_PSEAI Linoleate 9/13-lipoxygenase (Gene Name=lox)