Structure of PDB 4g1b Chain A

Receptor sequence

>4g1bA (length=398) Species: 307796 (Saccharomyces cerevisiae YJM789)

MLAEKTRSIIKATVPVLEQQGTVITRTFYKNMLTEHTELLNIFNRTNQKV
GAQPNALATTVLAAAKNIDDLSVLMDHVKQIGHKHRALQIKPEHYPIVGE
YLLKAIKEVLGDAATPEIINAWGEAYQAIADIFITVEKKMYEEALWPGWK
PFEITAKEYVASDIVEFTVKPKFGSGIELESLPITPGQYITVNTHPIRQE
NQYDALRHYSLCSASTKNGLRFAVKMEAARENFPAGLVSEYLHKDAKVGD
EIKLSAPAGDFAINKELIHQNEVPLVLLSSGVGVTPLLAMLEEQVKCNPN
RPIYWIQSSYDEKTQAFKKHVDELLAECANVDKIIVHTDTEPLINAAFLK
EKSPAHADVYTCGSLAFMQAMIGHLKELEHRDDMIHYEPFGPKMSTVQ

3D structure

• PDB: 4g1b Active site analysis of yeast flavohemoglobin based on its structure with a small ligand or econazole.
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 1.14.12.17: nitric oxide dioxygenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	I42 F43 N44 N47 T60 Q80 I81 K84 H85 L88 I90 H94 Y95 V98 F133 P392 K393 M394	I42 F43 N44 N47 T60 Q80 I81 K84 H85 L88 I90 H94 Y95 V98 F133 P392 K393 M394
BS02	FAD	A	T46 V50 K84 Y189 R207 H208 Y209 S210 A223 K225 E227 F233 P234 G236 L237 V238 S239 V282 F390	T46 V50 K84 Y189 R207 H208 Y209 S210 A223 K225 E227 F233 P234 G236 L237 V238 S239 V282 F390
BS03	ECN	A	F28 Y29 A56 L57 T60 L102 Y126	F28 Y29 A56 L57 T60 L102 Y126

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008941 nitric oxide dioxygenase NAD(P)H activity
• GO:0016491 oxidoreductase activity
• GO:0019825 oxygen binding
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0071949 FAD binding

Biological Process

• GO:0046210 nitric oxide catabolic process
• GO:0062197 cellular response to chemical stress
• GO:0071500 cellular response to nitrosative stress

External links

• PDB: RCSB:4g1b, PDBe:4g1b, PDBj:4g1b
• PDBsum: 4g1b
• PubMed: 23095020
• UniProt: A6ZUP2