Structure of PDB 4fxj Chain A

Receptor sequence
>4fxjA (length=498) Species: 9606 (Homo sapiens) [Search protein sequence]
EHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFS
HGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGA
EVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGL
ISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLK
FGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRF
DEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQM
LESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQNL
IAREAEAAIYHLQLFEELRRLAPDPTEATAVGAVEASFKCCSGAIIVLTK
SGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWA
EDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
3D structure
PDB4fxj M2 pyruvate kinase provides a mechanism for nutrient sensing and regulation of cell proliferation.
ChainA
Resolution2.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R73 R120 K270 T328
Catalytic site (residue number reindexed from 1) R46 R93 K240 T298
Enzyme Commision number 2.7.10.2: non-specific protein-tyrosine kinase.
2.7.11.1: non-specific serine/threonine protein kinase.
2.7.1.40: pyruvate kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PO4 A T432 K433 S434 S437 T399 K400 S401 S404
BS02 PO4 A H78 R120 H51 R93
BS03 PHE A R43 N44 N70 R106 H464 G468 I469 F470 R16 N17 N43 R79 H431 G435 I436 F437 PDBbind-CN: -logKd/Ki=3.62,IC50=240uM
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0003824 catalytic activity
GO:0004713 protein tyrosine kinase activity
GO:0004743 pyruvate kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0023026 MHC class II protein complex binding
GO:0030955 potassium ion binding
GO:0045296 cadherin binding
GO:0046872 metal ion binding
Biological Process
GO:0006096 glycolytic process
GO:0006417 regulation of translation
GO:0012501 programmed cell death
GO:0016310 phosphorylation
GO:0032869 cellular response to insulin stimulus
GO:0061621 canonical glycolysis
GO:1903672 positive regulation of sprouting angiogenesis
GO:2000767 positive regulation of cytoplasmic translation
Cellular Component
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005791 rough endoplasmic reticulum
GO:0005829 cytosol
GO:0005929 cilium
GO:0031982 vesicle
GO:0034774 secretory granule lumen
GO:0062023 collagen-containing extracellular matrix
GO:0070062 extracellular exosome
GO:1903561 extracellular vesicle
GO:1904813 ficolin-1-rich granule lumen

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4fxj, PDBe:4fxj, PDBj:4fxj
PDBsum4fxj
PubMed23530218
UniProtP14618|KPYM_HUMAN Pyruvate kinase PKM (Gene Name=PKM)

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