Structure of PDB 4fo6 Chain A

Receptor sequence

>4fo6A (length=315) Species: 9606 (Homo sapiens)

HNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACS
IPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQM
WYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQK
AAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGGIFSRLLDSLRQEG
FLTDDLVKGETKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYFTGSA
HFNRSMRALAKTKGMSLSEHALSTAVVRNTHGAKVGPGRVLPTPTEKDVF
RLLGLPYREPAERDW

3D structure

• PDB: 4fo6 The catalytic cycle for ribonucleotide incorporation by human DNA Pol lambda
• Chain: A
• Resolution: 2.007 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D176 D178 D230
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 K463 G464 T471 K472 R514 R517 K521 L527 S528 H530 H541	W23 V207 K208 G209 T211 K212 R254 R257 K261 L267 S268 H270 H281
BS02	dna	A	W342 G343 G345 K347 T348 R488 D490 Y505	W91 G92 G94 K96 T97 R228 D230 Y245
BS03	dna	A	Y267 W274 R275 Y279 P303 G304 G306 R308 M309 K312	Y16 W23 R24 Y28 P52 G53 G55 R57 M58 K61
BS04	dna	A	F499 P554	F239 P294
BS05	dna	A	Q400 L411 C412	Q149 L160 C161
BS06	MG	A	D427 D429	D176 D178
BS07	MN	A	D427 D429 D490	D176 D178 D230
BS08	F2A	A	R386 S417 R420 C425 G426 D427 D429 Y505 T507 G508 A510 N513	R135 S166 R169 C174 G175 D176 D178 Y245 T247 G248 A250 N253

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4fo6, PDBe:4fo6, PDBj:4fo6
• PDBsum: 4fo6
• PubMed: 22584622
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)