Structure of PDB 4flx Chain A

Receptor sequence

>4flxA (length=758) Species: 272844 (Pyrococcus abyssi GE5) [Search protein sequence]

HAGMIIDADYITEDGKPIIRIFKKEKGEFKVEYDRTFRPYIYALLKDDSA
IDEVKKITAERHGKIVRITEVEKVQKKFLGRPIEVWKLYLEHPQDVPAIR
EKIREHPAVVDIFEYDIPFAKRYLIDKGLTPMEGNEELTFLAVDIETLYH
EGEEFGKGPIIMISYADEEGAKVITWKSIDLPYVEVVSSEREMIKRLVKV
IREKDPDVIITYNGDNFAFPYLLKRAEKLGIKLPLGRDNSEPKMQRMGDS
LAVEIKGRIHFDLFPVIRRTINLPTYTLEAVYEAIFGKSKEKVYAHEIAE
AWETGKGLERVAKYSMEDAKVTFELGKEFFPMEAQLARLVGQPVWDVSRS
STGNLVEWFLLRKAYERNELAPNKPDEREYERRLRESYEGVKEPEKGLWE
GIVSLDFRSLYPSIIITHNVSPDTLNRENCKEYDVAPQVGHRFCKDFPGF
IPSLLGNLLEERQKIKKRMKESKDPVEKKLLDYRQRAIKILANSYYGYYG
YAKARWYCKECAESVTAWGRQYIDLVRRELESRGFKVLYIDTDGLYATIP
GAKHEEIKEKALKFVEYINSKLPGLLELEYEGFYARGFFVTKKKYALIDE
EGKIVTRGLEIVRRDWSEIAKETQAKVLEAILKHGNVDEAVKIVKEVTEK
LSKYEIPPEKLVIYEQITRPLSEYKAIGPHVAVAKRLAAKGVKVKPGMVI
GYIVLRGDGPISKRAIAIEEFDPKKHKYDAEYYIENQVLPAVERILRAFG
YRKEDLKY

3D structure

PDB

4flx Molecular Recognition of Canonical and Deaminated Bases by P. abyssi Family B DNA Polymerase.

Chain

Resolution

2.9 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Y7 P36 Y37 P90 Q91 P94 R97 Y112 D113 I114 P115 F116 R119 R243 G245 Y500 K502 K593 A675 I676 I710 S711 Y731	Y10 P39 Y40 P93 Q94 P97 R100 Y115 D116 I117 P118 F119 R122 R246 G248 Y501 K503 K594 A676 I677 I711 S712 Y732
BS02	dna	A	R265 R612 R613 D614 E664 Q665 T667 R668 Y673 K674 H679	R268 R613 R614 D615 E665 Q666 T668 R669 Y674 K675 H680
BS03	MG	A	D141 E143 D315	D144 E146 D318

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0071897	DNA biosynthetic process

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:4flx, PDBe:4flx, PDBj:4flx
PDBsum	4flx
PubMed	22902479
UniProt	P0CL77\|DPOL_PYRAB DNA polymerase 1 (Gene Name=polI)

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