Structure of PDB 4fl3 Chain A

Receptor sequence
>4fl3A (length=538) Species: 9606 (Homo sapiens) [Search protein sequence]
SANHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHG
RKAHHYTIERELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFN
RPQGVQPKTGPFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLI
ATTAHEKMPWFHGKISREESEQIVLIGSKTNGKFLIRARDNNGSYALCLL
HEGKVLHYRIDKDKTGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVLTVP
CQKREALPMDTEVFESPFADPEEIRPKEVYLDRKLLTLEDKELGSGNFGT
VKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQLDNPYIVR
MIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQVSMGMKY
LEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRAPVKWYAPECINYY
KFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPAGC
PREMYDLMNLCWTYDVENRPGFAAVELRLRNYYYDVVN
3D structure
PDB4fl3 Structural and biophysical characterization of the syk activation switch.
ChainA
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D494 A496 R498 N499 D512
Catalytic site (residue number reindexed from 1) D410 A412 R414 N415 D428
Enzyme Commision number 2.7.10.2: non-specific protein-tyrosine kinase.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 ANP A S379 G380 N381 V385 A400 K402 A451 P455 R498 L501 S295 G296 N297 V301 A316 K318 A367 P371 R414 L417 MOAD: Kd=150uM
PDBbind-CN: -logKd/Ki=3.82,Kd=150uM
BS02 MG A N499 D512 N415 D428
Gene Ontology
Molecular Function
GO:0001784 phosphotyrosine residue binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005102 signaling receptor binding
GO:0005178 integrin binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016170 interleukin-15 receptor binding
GO:0016301 kinase activity
GO:0019901 protein kinase binding
GO:0019902 phosphatase binding
GO:0035325 Toll-like receptor binding
GO:0042169 SH2 domain binding
GO:0043274 phospholipase binding
GO:0097110 scaffold protein binding
Biological Process
GO:0001525 angiogenesis
GO:0001775 cell activation
GO:0001819 positive regulation of cytokine production
GO:0001820 serotonin secretion
GO:0001945 lymph vessel development
GO:0002092 positive regulation of receptor internalization
GO:0002223 stimulatory C-type lectin receptor signaling pathway
GO:0002250 adaptive immune response
GO:0002281 macrophage activation involved in immune response
GO:0002283 neutrophil activation involved in immune response
GO:0002366 leukocyte activation involved in immune response
GO:0002554 serotonin secretion by platelet
GO:0002684 positive regulation of immune system process
GO:0002862 negative regulation of inflammatory response to antigenic stimulus
GO:0006468 protein phosphorylation
GO:0006606 protein import into nucleus
GO:0007159 leukocyte cell-cell adhesion
GO:0007166 cell surface receptor signaling pathway
GO:0007167 enzyme-linked receptor protein signaling pathway
GO:0007169 cell surface receptor protein tyrosine kinase signaling pathway
GO:0007229 integrin-mediated signaling pathway
GO:0009887 animal organ morphogenesis
GO:0010543 regulation of platelet activation
GO:0010803 regulation of tumor necrosis factor-mediated signaling pathway
GO:0016310 phosphorylation
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0019370 leukotriene biosynthetic process
GO:0019722 calcium-mediated signaling
GO:0030154 cell differentiation
GO:0030168 platelet activation
GO:0030183 B cell differentiation
GO:0030593 neutrophil chemotaxis
GO:0031334 positive regulation of protein-containing complex assembly
GO:0031623 receptor internalization
GO:0032481 positive regulation of type I interferon production
GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032752 positive regulation of interleukin-3 production
GO:0032753 positive regulation of interleukin-4 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032757 positive regulation of interleukin-8 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0032765 positive regulation of mast cell cytokine production
GO:0032928 regulation of superoxide anion generation
GO:0032930 positive regulation of superoxide anion generation
GO:0033630 positive regulation of cell adhesion mediated by integrin
GO:0035556 intracellular signal transduction
GO:0038063 collagen-activated tyrosine kinase receptor signaling pathway
GO:0038095 Fc-epsilon receptor signaling pathway
GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis
GO:0038156 interleukin-3-mediated signaling pathway
GO:0042492 gamma-delta T cell differentiation
GO:0042742 defense response to bacterium
GO:0043280 positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0043303 mast cell degranulation
GO:0043306 positive regulation of mast cell degranulation
GO:0043313 regulation of neutrophil degranulation
GO:0043366 beta selection
GO:0043410 positive regulation of MAPK cascade
GO:0045087 innate immune response
GO:0045579 positive regulation of B cell differentiation
GO:0045588 positive regulation of gamma-delta T cell differentiation
GO:0045780 positive regulation of bone resorption
GO:0046638 positive regulation of alpha-beta T cell differentiation
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0048514 blood vessel morphogenesis
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050764 regulation of phagocytosis
GO:0050776 regulation of immune response
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050853 B cell receptor signaling pathway
GO:0051090 regulation of DNA-binding transcription factor activity
GO:0051649 establishment of localization in cell
GO:0051712 positive regulation of killing of cells of another organism
GO:0070372 regulation of ERK1 and ERK2 cascade
GO:0071226 cellular response to molecule of fungal origin
GO:0071396 cellular response to lipid
GO:0071404 cellular response to low-density lipoprotein particle stimulus
GO:0071639 positive regulation of monocyte chemotactic protein-1 production
GO:0090237 regulation of arachidonate secretion
GO:0090330 regulation of platelet aggregation
GO:0097242 amyloid-beta clearance
GO:0120162 positive regulation of cold-induced thermogenesis
GO:1904263 positive regulation of TORC1 signaling
GO:1904646 cellular response to amyloid-beta
GO:1990858 cellular response to lectin
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0019815 B cell receptor complex
GO:0032009 early phagosome
GO:0032991 protein-containing complex
GO:0042101 T cell receptor complex

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Molecular Function

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Biological Process

View graph for
Cellular Component
External links
PDB RCSB:4fl3, PDBe:4fl3, PDBj:4fl3
PDBsum4fl3
PubMed23154170
UniProtP43405|KSYK_HUMAN Tyrosine-protein kinase SYK (Gene Name=SYK)

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