Structure of PDB 4fbw Chain A

Receptor sequence

>4fbwA (length=379) Species: 284812 (Schizosaccharomyces pombe 972h-)

NENTIRILISSDPHVGYGEKDPVRGNDSFVSFNEILEIARERDVDMILLG
GDIFHDNKPSRKALYQALRSLRLNCLGDKPCELELLSNINYLDPNINVAI
PVFSIHGNHDDRYSALDILQVTGLVNYFGRVPNIVVSPILLQKGFTKLAL
YGISNVRDERLYHSFRENKVKFLRPDLYRDEWFNLLTVHQNHSAHTPTSY
LPESFIQDFYDFVLWGHEHECLIDGSYNPTQKFTVVQPGSTIATSLSPGE
TAPKHCGILNITGKDFHLEKIRLRTVRPFIMKDIILSEVSSIPPMVENKK
EVLTYLISKVEEAITEANAQWYEAQGTVPVVENEKPPLPLIRLRVDYTGG
YQTENPQRFSNRFVGRVANATDVVQFYLK

3D structure

• PDB: 4fbw Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	K92 P93 C94 E95 L96 E97 Y116 L117 P119 N120 I121 N122 V123 Y195 F198 N201 V203 K204 F205 L206 R207 P208 D209 R212 Y233 S237 F238 Q240 D241 F242 Q264	K79 P80 C81 E82 L83 E84 Y91 L92 P94 N95 I96 N97 V98 Y162 F165 N168 V170 K171 F172 L173 R174 P175 D176 R179 Y200 S204 F205 Q207 D208 F209 Q231
BS02	MN	A	D65 N133 H222 H250	D52 N108 H189 H217
BS03	MN	A	D25 H27 D65 H252	D12 H14 D52 H219

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0008296 3'-5'-DNA exonuclease activity
• GO:0016787 hydrolase activity
• GO:0030145 manganese ion binding

Biological Process

• GO:0006302 double-strand break repair

Cellular Component

• GO:0005634 nucleus
• GO:0030870 Mre11 complex

External links

• PDB: RCSB:4fbw, PDBe:4fbw, PDBj:4fbw
• PDBsum: 4fbw
• PubMed: 22705791
• UniProt: Q09683|RAD32_SCHPO Double-strand break repair protein rad32 (Gene Name=rad32)