Structure of PDB 4f3o Chain A

Receptor sequence

>4f3oA (length=566) Species: 235909 (Geobacillus kaustophilus HTA426)

AKMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PATKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTANMRRQAKAVN
YGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGVKRYMENIVQEAKQ
KGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPIQGSAADIIKKAMI
DLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCRLVPEVMEQAVTLR
VPLKVDYHYGSTWYDA

3D structure

• PDB: 4f3o Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.
• Chain: A
• Resolution: 1.57 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T550 T552 T556 A558 R578 Q579 Y587 R615 Q624 N625 I626 P627 R629 V828 H829 D830	T254 T256 T260 A262 R282 Q283 Y291 R319 Q328 N329 I330 P331 R333 V519 H520 D521
BS02	dna	A	N527 N529 S530 K582 S585 T586 T611 Q612 T613 R615 S617 S618 T619 E620 N622 G711 Y714 I716 S717 Y719 G720 R771 F781 N782 R789 M790 N793 Q797	N231 N233 S234 K286 S289 T290 T315 Q316 T317 R319 S321 S322 T323 E324 N326 G402 Y405 I407 S408 Y410 G411 R462 F472 N473 R480 M481 N484 Q488
BS03	MN	A	Y654 S655 D830	Y358 S359 D521

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:4f3o, PDBe:4f3o, PDBj:4f3o
• PDBsum: 4f3o
• UniProt: Q5KWC1