Structure of PDB 4f2s Chain A

Receptor sequence

>4f2sA (length=578) Species: 235909 (Geobacillus kaustophilus HTA426) [Search protein sequence]

MAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFLRP
ETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLLAA
YLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEHLV
RKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVDTK
RLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPVLK
KTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVRPA
TKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESDWL
IFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDEVT
PNMRRQAKAVNYGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGVKR
YMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPIQG
SAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCRLV
PEVMEQAVTLRVPLKVDYHYGSTWYDAK

3D structure

PDB

4f2s Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.

Chain

Resolution

1.651 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	T550 K551 T552 T556 A558 R578 K582 Y587 R615 Q624 N625 I626 P627 I628 R629 H829 D830	T252 K253 T254 T258 A260 R280 K284 Y289 R317 Q326 N327 I328 P329 I330 R331 H531 D532
BS02	dna	A	N527 N529 S530 K582 S585 T586 T611 Q612 T613 R615 S617 S618 T619 E620 N622 Y710 G711 Y714 I716 S717 Y719 G720 N724 R771 F786 R789 M790 N793 Q797	N229 N231 S232 K284 S287 T288 T313 Q314 T315 R317 S319 S320 T321 E322 N324 Y412 G413 Y416 I418 S419 Y421 G422 N426 R473 F488 R491 M492 N495 Q499
BS03	GLC	A	E325 I605	E27 I307
BS04	FRU	A	D372 R375 D408 N607	D74 R77 D110 N309
BS05	CTP	A	R629 Q656 R702 K706 A707 Y710 Y714	R331 Q358 R404 K408 A409 Y412 Y416

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0008408	3'-5' exonuclease activity

	Biological Process
GO:0006139	nucleobase-containing compound metabolic process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:4f2s, PDBe:4f2s, PDBj:4f2s
PDBsum	4f2s
PubMed
UniProt	Q5KWC1

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