Structure of PDB 4ez9 Chain A

Receptor sequence

>4ez9A (length=578) Species: 235909 (Geobacillus kaustophilus HTA426)

KMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFLR
PETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLLA
AYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEHL
VRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVDT
KRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPVL
KKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVRP
ATKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESDW
LIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDEV
TPNMRRQAKAVNYGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGVK
RYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPIQ
GSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCRL
VPEVMEQAVTLRVPLKVDYHYGSTWYDA

3D structure

• PDB: 4ez9 Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.
• Chain: A
• Resolution: 1.64 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T550 K551 T552 T556 A558 R578 Q579 Y587 R615 Q624 N625 I626 P627 I628 R629 V828 H829 D830	T253 K254 T255 T259 A261 R281 Q282 Y290 R318 Q327 N328 I329 P330 I331 R332 V531 H532 D533
BS02	dna	A	N527 N529 S530 S585 T586 T611 Q612 T613 S617 S618 T619 E620 N622 Y714 I716 S717 Y719 N724 R771 R789 N793 Q797	N230 N232 S233 S288 T289 T314 Q315 T316 S320 S321 T322 E323 N325 Y417 I419 S420 Y422 N427 R474 R492 N496 Q500

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:4ez9, PDBe:4ez9, PDBj:4ez9
• PDBsum: 4ez9
• UniProt: Q5KWC1