Structure of PDB 4ex4 Chain A

Receptor sequence

>4ex4A (length=684) Species: 561304 (Mycobacterium leprae Br4923)

DRVSAGNLRVARVLYDFVNNEALPGTDINPNSFWSGVAKVVADLTPQNQS
LLNSRDELQAQIDKWHRHRVIFDVDAYRQFLIDIGYLLPEPDDFTISTSG
VDDEITMTAGPQLVVPVLNARFALNAANARWGSLYDALYGTDTIPETESE
YNKIRGDKVIAYARKFMDQAVPLASSWTNATGVSQLQIAASPEKFVGYNV
LLANHGLHIEVLIKDVILESAITTIMDFEDSVTAVDADDKVRGYRNWLGL
NKGDLTEEVNKDGKTFTRVLNADRSYTTPDGGELTLPGRSLLFVRNVGHL
TTSDAILVDGGDGQEKEVFEGIIDAVFTGLAAIHGLKTGEANGPLTNSRT
GSIYIVKPKMHGPAEVAFTCELFSRVEDVLGLPQGTLKVGIMDEERRTTL
NLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAGPMIRKGAMKNSTWIK
AYEDANVDIGLAAGFKGKAQIGKGMWAMTELMADMVEQKIGQPKAGATTA
WVPSPTAATLHAMHYHQVDVAAVQQELTGQRRATVDQLLTIPLAKKLAWA
PEEIREEVDNDCQSILGYVVRWVDQGIGCSKVPDIHNVALMEDRATLRIS
SQLLANWLRHGVITSEDVRASLERMAPLVDQQNAEDPAYRPMAPNFDDSI
AFLAAQELILSGAQQPNGYTEPILHRRRREFKAQ

3D structure

• PDB: 4ex4 The Structure of GlcB from Mycobacterium leprae
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D272 E274 R340 E439 D467 D638
• Catalytic site (residue number reindexed from 1): D227 E229 R295 E394 D422 D593
• Enzyme Commision number: 2.3.3.9: malate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	E439 D467	E394 D422
BS02	MG	A	N296 K297 H379 N387 L390	N251 K252 H334 N342 L345
BS03	MG	A	T391 N392	T346 N347

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004474 malate synthase activity
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006097 glyoxylate cycle
• GO:0006099 tricarboxylic acid cycle
• GO:0009436 glyoxylate catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:4ex4, PDBe:4ex4, PDBj:4ex4
• PDBsum: 4ex4
• UniProt: B8ZSN3|MASZ_MYCLB Malate synthase G (Gene Name=glcB)