Structure of PDB 4ef3 Chain A

Receptor sequence
>4ef3A (length=466) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
ERPTLPIPDLLTTDARNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVK
LQRGKAVTVDIYNQLTEETTLHWHGLEVPGEVDGGPQGIIPPGGKRSVTL
NVDQPAATCWFHPHQHGKTGRQVAMGLAGLVVIEDDEILKLMLPKQWGID
DVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNGAIYPQHAAPRG
WLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVLMGE
RFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGA
LPDTLSSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGM
FDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGTQ
FRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCH
LLEHEDTGMMLGFTVG
3D structure
PDB4ef3 Exogenous acetate ion reaches the type II copper centre in CueO through the water-excretion channel and potentially affects the enzymatic activity.
ChainA
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H101 H103 H141 H143
Catalytic site (residue number reindexed from 1) H72 H74 H112 H114
Enzyme Commision number 1.16.3.4: cuproxidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CU A H443 C500 H505 H392 C449 H454
BS02 CU A H103 H141 H501 H74 H112 H450
BS03 CU A H143 H448 H499 H114 H397 H448
BS04 CU A H101 H103 H446 H448 H72 H74 H395 H397
BS05 O A H101 H103 H446 H448 H72 H74 H395 H397
Gene Ontology
Molecular Function
GO:0004322 ferroxidase activity
GO:0005507 copper ion binding
GO:0016491 oxidoreductase activity
GO:0016682 oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
GO:0016722 oxidoreductase activity, acting on metal ions
GO:0016724 oxidoreductase activity, acting on metal ions, oxygen as acceptor
GO:0046872 metal ion binding
Biological Process
GO:0010273 detoxification of copper ion
GO:0046688 response to copper ion
Cellular Component
GO:0030288 outer membrane-bounded periplasmic space
GO:0042597 periplasmic space

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Cellular Component
External links
PDB RCSB:4ef3, PDBe:4ef3, PDBj:4ef3
PDBsum4ef3
PubMed27380373
UniProtP36649|CUEO_ECOLI Multicopper oxidase CueO (Gene Name=cueO)

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