Structure of PDB 4eey Chain A

Receptor sequence

>4eeyA (length=426) Species: 9606 (Homo sapiens)

ATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVS
YEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVME
IMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLP
QGPTEETVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAA
IERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIR
KIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCR
GIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLT
KDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVI
KNMNTSTEWSPPLTMLFLCATKFSAS

3D structure

• PDB: 4eey Structural basis for cisplatin DNA damage tolerance by human polymerase {eta} during cancer chemotherapy.
• Chain: A
• Resolution: 2.32 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 I48 S62 K86 R93 K293 R313 P316 K317 T318 G320 C321 S322 K323 N324	Q37 Y38 I47 S61 K85 R92 K290 R310 P313 K314 T315 G317 C318 S319 K320 N321
BS02	dna	A	E116 K224 S257 G259 G260 K261 L262 R382 R383 C384	E115 K221 S254 G256 G257 K258 L259 R379 R380 C381
BS03	DCP	A	M14 D15 C16 F17 F18 Y52 R55 R61 D115 K231	M13 D14 C15 F16 F17 Y51 R54 R60 D114 K228
BS04	MG	A	D13 M14 D115	D12 M13 D114

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4eey, PDBe:4eey, PDBj:4eey
• PDBsum: 4eey
• PubMed: 22562137
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)