Structure of PDB 4ecw Chain A

Receptor sequence

>4ecwA (length=432) Species: 9606 (Homo sapiens)

GPHMATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGI
IAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASV
EVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYI
EGLPQGPEETVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEM
RAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQM
PIRKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYA
MCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEE
RLTKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAF
TVIKNCNTSGIQTEWSPPLTMLFLCATKFSAS

3D structure

• PDB: 4ecw Watching DNA polymerase eta make a phosphodiester bond
• Chain: A
• Resolution: 1.896 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 W42 R61 S62 K86 R93 P316 K317 T318 G320 S322 K323 N324 P326 G327 R351	Q41 Y42 W45 R64 S65 K89 R96 P316 K317 T318 G320 S322 K323 N324 P326 G327 R351
BS02	dna	A	F18 R61 D115 E116 K224 S257 G259 G260 K261 L262 R377 R382 C384	F21 R64 D118 E119 K224 S257 G259 G260 K261 L262 R377 R382 C384
BS03	MG	A	D13 D115 E116	D16 D118 E119
BS04	CA	A	D13 M14 D115	D16 M17 D118
BS05	MG	A	D13 M14 D115	D16 M17 D118
BS06	DTP	A	D13 M14 D15 C16 F17 F18 Y52 R55 R61 D115 K231	D16 M17 D18 C19 F20 F21 Y55 R58 R64 D118 K231

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4ecw, PDBe:4ecw, PDBj:4ecw
• PDBsum: 4ecw
• PubMed: 22785315
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)