Structure of PDB 4ecs Chain A

Receptor sequence

>4ecsA (length=430) Species: 9606 (Homo sapiens)

GPHMATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGI
IAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASV
EVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYI
EGLPQGPTVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRA
AIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPI
RKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMC
RGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERL
TKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTV
IKNCNTSGIQTEWSPPLTMLFLCATKFSAS

3D structure

• PDB: 4ecs Watching DNA polymerase eta make a phosphodiester bond
• Chain: A
• Resolution: 1.951 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D13 M14 Y52 R55 R61 S113 D115 E116 K231
• Catalytic site (residue number reindexed from 1): D16 M17 Y55 R58 R64 S116 D118 E119 K229
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 W42 S62 K86 L89 R93 K293 P316 K317 T318 G320 C321 S322 K323 N324 P326 G327 R351	Q41 Y42 W45 S65 K89 L92 R96 K291 P314 K315 T316 G318 C319 S320 K321 N322 P324 G325 R349
BS02	dna	A	F18 R61 S113 D115 E116 K224 S257 G259 K261 L262 R377 L381 R382 C384	F21 R64 S116 D118 E119 K222 S255 G257 K259 L260 R375 L379 R380 C382
BS03	MG	A	D13 D115 E116	D16 D118 E119
BS04	CA	A	D13 M14 D115	D16 M17 D118
BS05	MG	A	D13 M14 D115	D16 M17 D118
BS06	DTP	A	D13 M14 D15 C16 F17 F18 Y52 R55 R61 D115 K231	D16 M17 D18 C19 F20 F21 Y55 R58 R64 D118 K229

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4ecs, PDBe:4ecs, PDBj:4ecs
• PDBsum: 4ecs
• PubMed: 22785315
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)