Structure of PDB 4ebc Chain A

Receptor sequence

>4ebcA (length=376) Species: 9606 (Homo sapiens)

SSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGV
KKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGF
DENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLV
GSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPES
CQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGI
SVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELL
ASLLNRVCQDRKPHTVRLIIRRYGRESRQCPIPSHVIQKDVMTPMVDILM
KLFRNMVNVKMPFHLTLLSVCFCNLK

3D structure

• PDB: 4ebc A Nucleotide-Analogue-Induced Gain of Function Corrects the Error-Prone Nature of Human DNA Polymerase iota.
• Chain: A
• Resolution: 2.901 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E127 K207 I242 G243 K245 T246 E358 S359 R360 Q361	E102 K182 I217 G218 K220 T221 E326 S327 R328 Q329
BS02	dna	A	K60 Y61 L62 L99 Q300 S301 S303 E304 E305 S307 R331	K35 Y36 L37 L74 Q275 S276 S278 E279 E280 S282 R306
BS03	0OH	A	C37 F38 Y39 T65 Y68 R71 K77 L78 D126	C12 F13 Y14 T40 Y43 R46 K52 L53 D101
BS04	CA	A	D34 L35 D126	D9 L10 D101
BS05	CA	A	K237 I239	K212 I214
BS06	CA	A	D34 E127	D9 E102

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4ebc, PDBe:4ebc, PDBj:4ebc
• PDBsum: 4ebc
• PubMed: 22632140
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)