Structure of PDB 4e2y Chain A

Receptor sequence

>4e2yA (length=405) Species: 1874 (Micromonospora chalcea)

PTACRVCGGGVQEFLDLGRQPLSDRFRKPDELDDEFTYRLAVGRCDSCEM
VQLTEEVPRDLMFHEVYPYHSSGSSVMREHFAMLARDFLATELTGPDPFI
VEIGCNDGIMLRTIQEAGVRHLGFEPSSGVAAKAREKGIRVRTDFFEKAT
ADDVRRTEGPANVIYAANTLCHIPYVQSVLEGVDALLAPDGVFVFEDPYL
GDIVAKTSFDQIYDQHFFLFSATSVQGMAQRCGFELVDVQRLPVHGGEVR
YTLARQGSRTPSAAVAQLLAAEREQELSDMATLRAFAGNVVKIRDELTAL
LHRLRAEGRSVVGYGATAKSATVTNFCGIGPDLVHSVYDTTPDKQNRLTP
GAHIPVRPASAFSDPYPDYALLFAWNHAEEIMAKEQEFHQAGGRWILYVP
EVHIR

3D structure

• PDB: 4e2y Probing the catalytic mechanism of a C-3'-methyltransferase involved in the biosynthesis of D-tetronitrose.
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C13 C16 C54 C57	C4 C7 C45 C48
BS02	SAH	A	F72 Y76 S80 I112 G113 N115 F133 E134 P135 S136 F154 F155 A176 N177 T178 I182	F63 Y67 S71 I103 G104 N106 F124 E125 P126 S127 F145 F146 A167 N168 T169 I173
BS03	JHZ	A	Y78 H181 Y222 H254 T326 A327 K328 D348 T349 N385 H386 E389 I390 K393	Y69 H172 Y213 H245 T317 A318 K319 D339 T340 N376 H377 E380 I381 K384

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:4e2y, PDBe:4e2y, PDBj:4e2y
• PDBsum: 4e2y
• PubMed: 22495991
• UniProt: B5L6K6