Structure of PDB 4dm0 Chain A

Receptor sequence

>4dm0A (length=460) Species: 562 (Escherichia coli)

TSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKSITISSEG
SKAAQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPD
DPADADEKESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLA
HNERFVVRSKHPRKDVESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGK
RKNRPARKASLSLRSGRITLKQGNITLNAVLAEEINPPKGETPLKWLLLT
SEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAERQRMEKPDNLERM
VSILSFVAVRLLQLRESFTPVESQSAETVLTPDECQLLGYLDKGKRKRKE
KAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQSKLDGFLAAK
DLMAQGIKIG

3D structure

• PDB: 4dm0 Two-metal active site binding of a TN5 transposase synaptic complex
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T99 Q243 K244 G245 R253 N255 H329 K330 K333 T334 K439 R440	T97 Q241 K242 G243 R251 N253 H327 K328 K331 T332 K422 R423
BS02	dna	A	R210 Y237 I239 K244 R256 W298 Y319 R322 W323 E326 R367 F435 S438 K439 I443 S445	R208 Y235 I237 K242 R254 W296 Y317 R320 W321 E324 R365 F418 S421 K422 I426 S428
BS03	MN	A	D97 E326	D95 E324
BS04	MN	A	D97 D188	D95 D186
BS05	MN	A	S178 S181	S176 S179

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004803 transposase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006310 DNA recombination
• GO:0006313 DNA transposition
• GO:0032196 transposition

External links

• PDB: RCSB:4dm0, PDBe:4dm0, PDBj:4dm0
• PDBsum: 4dm0
• PubMed: 11896402
• UniProt: Q46731|TN5P_ECOLX Transposase for transposon Tn5 (Gene Name=tnpA)