Structure of PDB 4dlb Chain A

Receptor sequence

>4dlbA (length=379) Species: 4081 (Solanum lycopersicum)

SATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKVLYTALCHTD
AYTWSGKDPEGLFPCVLGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECR
ECKFCKSGKTNLCGKVRAATGVGVMMNDRKSRFSINGKPIYHFMGTSTFS
QYTVVHDVSVAKIDPVAPLEKVCLLGCGVPTGLGAVWNTAKVEPGSIVAV
FGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFDRAKNFGVTEFINPKEHE
QPIQQVIVDLTDGGVDYSFECIGNVSVMRSALECCHKGWGTSVIVGVAAS
GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPSLVDKYLKKEIKVDEYIT
HNMTLADINKAFDLMHDGDCLRVVLDMFV

3D structure

• PDB: 4dlb Structural and functional characterization of a plant S-nitrosoglutathione reductase from Solanum lycopersicum.
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C47 H48 T49 Y52 H69 E70 C99 C102 C105 C113 R117 C177 T181 R372
• Catalytic site (residue number reindexed from 1): C47 H48 T49 Y52 H69 E70 C99 C102 C105 C113 R117 C177 T181 R372
• Enzyme Commision number: 1.1.1.284: S-(hydroxymethyl)glutathione dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C47 H69 C177	C47 H69 C177
BS02	ZN	A	C99 C102 C105 C113	C99 C102 C105 C113
BS03	NAD	A	Y95 C177 T181 G204 V206 D226 I227 K231 C271 I272 V295 V297 A321	Y95 C177 T181 G204 V206 D226 I227 K231 C271 I272 V295 V297 A321

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004022 alcohol dehydrogenase (NAD+) activity
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051903 S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity

Biological Process

• GO:0044281 small molecule metabolic process
• GO:0046294 formaldehyde catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:4dlb, PDBe:4dlb, PDBj:4dlb
• PDBsum: 4dlb
• PubMed: 23274177
• UniProt: D2Y3F4