Structure of PDB 4dg8 Chain A

Receptor sequence

>4dg8A (length=495) Species: 208964 (Pseudomonas aeruginosa PAO1)

QNSLLDLYAHPTVVARFSEMAALHPHREAIRDRFGSVDYRQLLDSAEQLS
DYLLEHYPQPGVCLGVYGEYSRESITCLLAILLSGHHYLYIDLKQPAAWN
AELCRQVDCRLILDCSTTPTPANGLPCVPVRHLPAAPASVARPCFAADQI
AYINFSSGTTGRPKAIACTHAGITRLCLGQSFLAFAPQMRFLVNSPLSFD
AATLEIWGALLNGGCCVLNDLGPLDPGVLRQLIGERGADSAWLTASLFNT
LVDLDPDCLGGLRQLLTGGDILSVPHVRRALLRHPRLHLVNGYGPTENTT
FTCCHVVTDDDLEEDDIPIGKAIAGTAVLLLDEHGQEIAEPDRAGEIVAF
GAGLAQGYRNDAARTRASFVELPYRGRLLRAYRTGDRARYDEQGRLRFIG
RGDGQVKLNGYRLDLPALEQRFRRQPGILDCALLVRERNGVKQLLCAWTG
KADASPQALLRQLPTWQRPHACVRVEALPLTLDRAALLRRLEEPL

3D structure

• PDB: 4dg8 Structure of PA1221, a Nonribosomal Peptide Synthetase Containing Adenylation and Peptidyl Carrier Protein Domains.
• Chain: A
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S170 E311 K421 R426
• Catalytic site (residue number reindexed from 1): S156 E297 K407 R412
• Enzyme Commision number: 6.2.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AMP	A	G283 D284 I285 G306 Y307 G308 P309 T310 D400 F412 K421 R426	G269 D270 I271 G292 Y293 G294 P295 T296 D386 F398 K407 R412
BS02	BU3	A	W256 G283	W242 G269
BS03	BU3	A	S209 W256	S195 W242

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0031177 phosphopantetheine binding

Biological Process

• GO:0043041 amino acid activation for nonribosomal peptide biosynthetic process
• GO:0044550 secondary metabolite biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4dg8, PDBe:4dg8, PDBj:4dg8
• PDBsum: 4dg8
• PubMed: 22452656
• UniProt: Q9I4B7