Structure of PDB 4dcm Chain A

Receptor sequence

>4dcmA (length=360) Species: 83333 (Escherichia coli K-12)

MRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEIRGPVLILNDAFGA
LSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTADYPQQP
GVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEK
VLGPTTTTLAWKKARLINCTFNEPQLADAPQTVSWKLEGTDWTIHNHANV
FSRTGLDIGARFFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVF
VDESPMAVASSRLNVETNMPEALDRCEFMINNALSGVEPFRFNAVLCNPP
FDNVAWEMFHHARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNN
KFVVLKAVKL

3D structure

• PDB: 4dcm Structural insights into the function of 23S rRNA methyltransferase RlmG (m2G1835) from Escherichia coli.
• Chain: A
• Resolution: 2.297 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.174: 23S rRNA (guanine(1835)-N(2))-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAM	A	F208 G236 C237 D259 E260 N289 A290 N305 M322	F201 G229 C230 D252 E253 N282 A283 N298 M308

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0008170 N-methyltransferase activity
• GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
• GO:0008990 rRNA (guanine-N2-)-methyltransferase activity
• GO:0052916 23S rRNA (guanine(1835)-N(2))-methyltransferase activity

Biological Process

• GO:0006364 rRNA processing
• GO:0031167 rRNA methylation
• GO:0032259 methylation
• GO:0043414 macromolecule methylation
• GO:0070475 rRNA base methylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4dcm, PDBe:4dcm, PDBj:4dcm
• PDBsum: 4dcm
• PubMed: 22753782
• UniProt: P42596|RLMG_ECOLI Ribosomal RNA large subunit methyltransferase G (Gene Name=rlmG)