Structure of PDB 4d57 Chain A

Receptor sequence

>4d57A (length=482) Species: 1160 (Planktothrix agardhii)

FKQSIHQLFETQVERTPEAVAVLSEQGQLTYEELNTKANQLAHYLRTLGV
KSETLVGVCVDRSLEMVIGLLAILKAGGAYVPLDPTYPRERLTYMVQDAQ
ISVLVTQTQWSNLISDYQGQVICLDSQWAKIASYSQENLVNTVNPENLAY
VIYTSGSTGKPKGVMIEHQSLVNFTKLAIAQYQITTSDRTLQFVSISFDV
AAEEIYVTLCSGATLILRTEEMISSIPSFVQKSQDWQITVWSLPTAYWHL
LVNELVKSKIALPDSLRLVIIGGERVQPELVRMWFKNVGNFPELINVYGP
TEGTIAVSLCRLSQLTESQRNRTEIPIGKSLGENISVYVLDETLKTVPPE
TPGEIYIGGTALARGYLNRPELTAQKFIQDPFSPSERLYKTGDLGRYLAD
GNLEYLGRVDHQVKINGFRVELGEIETVLLQHHQVAQAVVIDRRLVAYLV
PHSTEENLTVTLQQFLKNKLPSYMIPATFVVL

3D structure

• PDB: 4d57 Structural Elucidation of the Bispecificity of a Domains as a Basis for Activating Non-Natural Amino Acids.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T155 F175 T302 E303 K415 R420
• Catalytic site (residue number reindexed from 1): T154 F174 T301 E302 K414 R419

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ARG	A	D200 V201 E204 V298 G300 I306	D199 V200 E203 V297 G299 I305
BS02	AMP	A	G274 E275 R276 V298 Y299 G300 P301 T302 D394 K415 R420	G273 E274 R275 V297 Y298 G299 P300 T301 D393 K414 R419

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0031177 phosphopantetheine binding
• GO:0046872 metal ion binding

Biological Process

• GO:0043041 amino acid activation for nonribosomal peptide biosynthetic process
• GO:0044550 secondary metabolite biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:4d57, PDBe:4d57, PDBj:4d57
• PDBsum: 4d57
• PubMed: 26096082
• UniProt: G0WVH3