Structure of PDB 4cxr Chain A

Receptor sequence

>4cxrA (length=425) Species: 1773 (Mycobacterium tuberculosis)

LTPEQIIAVDGAHLWHPYSSIGRSPVVAVAAHGAWLTLIRDGQPIEVLDA
MSSWWTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKLLVDI
TPAGLDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGGYHGD
TFLAMSICDPHGGMHSLWTDVLAAQVFAPQVPRDYDPAYSAAFEAQLAQH
AGELAAVVVEPVVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEIATGF
GRTGALFAADHAGVSPDIMCVGKALTGGYLSLAATLCTADVAHTISAGAA
GALMHGPTFMANPLACAVSVASVELLLGQDWRTRITELAAGLTAGLDTAR
ALPAVTDVRVCGAIGVIECDRPVDLAVATPAALDRGVWLRPFRNLVYAMP
PYICTPAEITQITSAMVEVARLVGS

3D structure

• PDB: 4cxr Inhibition of Mycobacterium Tuberculosis Transaminase Bioa by Aryl Hydrazines and Hydrazides.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y25 Y157 E220 D254 A257 K283 Y407
• Catalytic site (residue number reindexed from 1): Y18 Y147 E210 D244 A247 K273 Y397
• Enzyme Commision number: 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	A	G124 S125 Y157 H158 E220 D254 I256 K283	G114 S115 Y147 H148 E210 D244 I246 K273
BS02	2BG	A	Y25 W64 W65 A226	Y18 W54 W55 A216
BS03	PLP	A	P317 T318	P307 T308

Gene Ontology

Molecular Function

• GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
• GO:0008483 transaminase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0009102 biotin biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4cxr, PDBe:4cxr, PDBj:4cxr
• PDBsum: 4cxr
• PubMed: 24482078
• UniProt: P9WQ81|BIOA_MYCTU Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)