Structure of PDB 4cmq Chain A

Receptor sequence
>4cmqA (length=1156) Species: 301447 (Streptococcus pyogenes serotype M1) [Search protein sequence]
KYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLF
DSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLER
HPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFR
GHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSAR
LSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQ
LSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRKAPLSASM
IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEE
FYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHA
ILRRQEDFYPFLKDNREKIEKILTFRPNEKVLPKHSLLYEYFTVYNELTK
VKYVTEFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKEISGVEDRFNAS
LGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYA
HLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKTILDFLKRNFMQLIHDD
SLTFKEDIQKAQVSDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMAREQKNSRERMKRIEEGIKELHPVENTQLQNEKLYLYYLQ
NGRDMYVDQELDINVDHIVPQSFLKDDSIDNKVLTRNRGKSDNVPSEEVV
KKMKNYWRQLLNAKLITQRKFDNLTKLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY
HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEYFFYS
NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
QVNIVKKTEVQTPTVAYSVLVVSVKELLGITIMERSSFEKNPIDFLEAKG
KDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASH
YVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAE
NIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYE
TRIDLS
3D structure
PDB4cmq Structures of Cas9 Endonucleases Reveal RNA- Mediated Conformational Activation
ChainA
Resolution3.09 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MN A D10 E766 H983 D7 E663 H852
BS02 MN A D10 E762 E766 D7 E659 E663
BS03 MN A H511 E516 H435 E440
BS04 MN A H1297 D1328 H1090 D1121
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:4cmq, PDBe:4cmq, PDBj:4cmq
PDBsum4cmq
PubMed24505130
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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