Structure of PDB 4cis Chain A

Receptor sequence
>4cisA (length=263) Species: 1359 (Lactococcus cremoris) [Search protein sequence]
PELPEVETVRRELEKRIVGQKIISIEATYPRMVLTGFEQLKKELTGKIIQ
GIIRRGKYLIFEIGDDFRLISHLRMEGKYRLATLDAPREKHDHLTMKFSD
GQLIYADVRKFGTWELISTDQVLPYFLNKKIGPEPTYEDFDEKLFREKLR
KSTKKIKPYLLEQTLVAGLGNIYVDEVLWLAKIHPEKEANQLIESSIHLL
HDSIIEILQKAIKLGGSSITGKMQNELQVYGKTGEKCSRCGAEIQKIKVA
GRGTHFCPFCQQK
3D structure
PDB4cis Ribose-protonated DNA base excision repair: a combined theoretical and experimental study.
ChainA
Resolution2.05 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) P1 E2
Catalytic site (residue number reindexed from 1) P1 E2
Enzyme Commision number 3.2.2.23: DNA-formamidopyrimidine glycosylase.
4.2.99.18: DNA-(apurinic or apyrimidinic site) lyase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A F111 K130 F111 K130
BS02 dna A K57 H72 R74 M75 K129 L161 E162 Q163 G170 R260 K57 H72 R74 M75 K129 L161 E162 Q163 G170 R252
BS03 ZN A C245 C248 C265 C268 C237 C240 C257 C260
BS04 BU3 A Y58 H72 R74 T113 K129 Y58 H72 R74 T113 K129
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003684 damaged DNA binding
GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity
GO:0008270 zinc ion binding
GO:0008534 oxidized purine nucleobase lesion DNA N-glycosylase activity
GO:0016787 hydrolase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0016799 hydrolase activity, hydrolyzing N-glycosyl compounds
GO:0016829 lyase activity
GO:0019104 DNA N-glycosylase activity
GO:0034039 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity
GO:0046872 metal ion binding
GO:0140078 class I DNA-(apurinic or apyrimidinic site) endonuclease activity
Biological Process
GO:0006281 DNA repair
GO:0006284 base-excision repair

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Molecular Function
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Biological Process
External links
PDB RCSB:4cis, PDBe:4cis, PDBj:4cis
PDBsum4cis
PubMed25065673
UniProtQ031W6

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