Structure of PDB 4cfh Chain A

Receptor sequence

>4cfhA (length=402) Species: 10116 (Rattus norvegicus)

RVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVG
KIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNG
RLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFG
LSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLC
GTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATI
KDIREHEWFKQDLPKYLFPEDAVAYHLIIDNRRIMNEAKDFYLATSPPDS
FLDDHHLTRPHPERVPFLVAETPRAAKWHLGIRSQSRPNDIMAEVCRAIK
QLDYEWKVVNPYYLRVRRKNPVTSTFSKMSLQLYQVDSRTYLLDFRSIDD
EI

3D structure

• PDB: 4cfh Structure of Mammalian Ampk and its Regulation by Adp
• Chain: A
• Resolution: 3.24 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D139 K141 E143 N144 D157 S176
• Catalytic site (residue number reindexed from 1): D130 K132 E134 N135 D148 S167
• Enzyme Commision number: 2.7.11.1: non-specific serine/threonine protein kinase.
  2.7.11.26: [tau protein] kinase.
  2.7.11.31: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	R415 A416 Q419 Y422 R436 S445 M447 R464 S465	R347 A348 Q351 Y354 R368 S377 M379 R396 S397
BS02	STU	A	L22 G23 G25 A43 K45 M93 E94 Y95 V96 G99 E100 E143 N144 L146 D157	L13 G14 G16 A34 K36 M84 E85 Y86 V87 G90 E91 E134 N135 L137 D148

Gene Ontology

Molecular Function

• GO:0004672 protein kinase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006468 protein phosphorylation

External links

• PDB: RCSB:4cfh, PDBe:4cfh, PDBj:4cfh
• PDBsum: 4cfh
• PubMed: 21399626
• UniProt: P54645|AAPK1_RAT 5'-AMP-activated protein kinase catalytic subunit alpha-1 (Gene Name=Prkaa1)