Structure of PDB 4cei Chain A

Receptor sequence

>4ceiA (length=1170) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

KPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENP
IDVDRLLVVTFTNASAAEMKHRIAEALEKELVQRPGSLHIRRQLSLLNRA
SISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELIGDEVLDELFEDEY
AKGEKAFFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHL
YDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRALELTKAPGGPAPRA
DNFLDDLAQIDELIQHQDDFSELYKRVPAVSFKRAKAVKGDEFDPALLDE
ATDLRNGAKKLLEKLKTDYFTRSPEQHLKSLAEMKPVIETLVQLVISYGK
RFEAAKQEKSIIDFSDLEHYCLAILTAENDKGEREPSEAARFYQEQFHEV
LVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLF
LSKYKRFTESGEGTGRKIDLNKNFRSRADILDSTNFLFKQLMGGKIGEVD
YDEQAELKLGAAYPDNDETETELLLIDNAEELETVQFEAKAIAKEIRKLI
SSPFKVYDGKKKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANL
TSGYFEAVEVAVALSVLKVIDNPYQDIPLASVLRSPIVGADENELSLIRL
ENKKAPYYEAMKDYLAAGDRSDELYQKLNTFYGHLQKWRAFSKNHSVSEL
IWEVYRDTKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLR
FIERMQERGDDLGTARGLSEQEDVVRLMTIHSSKGLEFPVVFVAGLGRNF
NMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEE
LRVLYVALTRAKEKLFLIGSCKDHQKQLAKWQASASQTDWLLPEFDRYQA
RTYLDFIGPALARHRDLHADISGHPARFAVQMIHSYDLLEKSERLEAIRR
GEPVPGSFAFDEKAREQLSWTYPHQEVTQIRTKQSVSEILYRRPAFMMKK
GLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVHRLYEKELLTEEQKDAI
DIEEIVQFFHTEIGGQLIGAKWKDREIPFSLALPAKEIYPDAHEADEPLL
VQGIIDCLYETEDGLYLLAYKSDRIEAAPILKKRYETQIQLYTKAVEQIA
KTKVKGCALYFFDGGHILTL

3D structure

• PDB: 4cei Structural Basis for Translocation by Addab Helicase-Nuclease and its Arrest at Chi Sites.
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K36 T37 D407 E408 Q441 R873
• Catalytic site (residue number reindexed from 1): K32 T33 D403 E404 Q437 R860
• Enzyme Commision number: 3.1.-.-
  5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	F65 N67 T108 H110 S111 I132 Q135 R288 A289 K290 F368 Y444 F446 R590 S591 H794 N812 N814 R914	F61 N63 T104 H106 S107 I128 Q131 R284 A285 K286 F364 Y440 F442 R577 S578 H781 N799 N801 R901
BS02	ANP	A	T10 Q15 G33 S34 G35 K36 T37 A38 R76 Q441 F478 R479 K573 E800 R873	T6 Q11 G29 S30 G31 K32 T33 A34 R72 Q437 F474 R475 K560 E787 R860

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003678 DNA helicase activity
• GO:0003690 double-stranded DNA binding
• GO:0004386 helicase activity
• GO:0004527 exonuclease activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008408 3'-5' exonuclease activity
• GO:0016787 hydrolase activity
• GO:0016853 isomerase activity
• GO:0016887 ATP hydrolysis activity
• GO:0043138 3'-5' DNA helicase activity

Biological Process

• GO:0000724 double-strand break repair via homologous recombination
• GO:0000725 recombinational repair
• GO:0006281 DNA repair
• GO:0006302 double-strand break repair
• GO:0032508 DNA duplex unwinding

Cellular Component

• GO:0005829 cytosol
• GO:0033202 DNA helicase complex

External links

• PDB: RCSB:4cei, PDBe:4cei, PDBj:4cei
• PDBsum: 4cei
• PubMed: 24670664
• UniProt: P23478|ADDA_BACSU ATP-dependent helicase/nuclease subunit A (Gene Name=addA)