Structure of PDB 4c8l Chain A

Receptor sequence

>4c8lA (length=540) Species: 271 (Thermus aquaticus)

ALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKAL
RDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVAR
RYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLA
HMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLER
VLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLK
STYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQ
RIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTET
ASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAF
IERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVRE
AAERMAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAP
KERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

3D structure

• PDB: 4c8l Structural Insights Into DNA Replication without Hydrogen Bonds.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R487 T506 E507 K508 T509 S513 T514 S515 A516 R536 K540 R573 Q582 N583 P585 V586 R587 Y671 N750 Q754 H784	R195 T214 E215 K216 T217 S221 T222 S223 A224 R244 K248 R281 Q290 N291 P293 V294 R295 Y379 N458 Q462 H492
BS02	dna	A	N483 N485 S486 S543 S577 R587 F667 Y671 M673 R677 R746 M747	N191 N193 S194 S251 S285 R295 F375 Y379 M381 R385 R454 M455

Gene Ontology

Molecular Function

• GO:0001882 nucleoside binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair

External links

• PDB: RCSB:4c8l, PDBe:4c8l, PDBj:4c8l
• PDBsum: 4c8l
• PubMed: 24283923
• UniProt: P19821|DPO1_THEAQ DNA polymerase I, thermostable (Gene Name=polA)