Structure of PDB 4c2t Chain A

Receptor sequence

>4c2tA (length=650) Species: 1299 (Deinococcus radiodurans) [Search protein sequence]

AGPDLLQALNPTQAQAADHFTGPALVIAGAGSGKTRTLIYRIAHLIGHYG
VHPGEILAVTFTNKAAAEMRERAGHLVPGAGDLWMSTFHSAGVRILRTYG
EHIGLRRGFVIYDDDDQLDIIKEVMGSIPGIGAETQPRVIRGIIDRAKSN
LWTPDDLDRSREPFISGLPRDAAAEAYRRYEVRKKGQNAIDFGDLITETV
RLFKEVPGVLDKVQNKAKFIHVDEYQDTNRAQYELTRLLASRDRNLLVVG
DPDQSIYKFRGADIQNILDFQKDYPDAKVYMLEHNYRSSARVLEAANKLI
ENNTERLDKTLKPVKEAGQPVTFHRATDHRAEGDYVADWLTRLHGEGRAW
SEMAILYRTNAQSRVIEESLRRVQIPARIVGGVGFYDRREIRDILAYARL
ALNPADDVALRRIIGRPRRGIGDTALQKLMEWARTHHTSVLTACANAAEQ
NILDRGAHKATEFAGLMEAMSEAADNYEPAAFLRFVMETSGYLDLLRQEG
QEGQVRLENLEELVSAAEEWSQDEANVGGSIADFLDDAALLSSDMRTKAE
DAVTLMTLHNAKGLEFPVVFIVGVEQGLLPSKGAIAEGPSGIEEERRLFY
VGITRAMERLLMTAAQNRMQFGKTNAAEDSAFLEDIEGLFDTVDPYGQPI

3D structure

PDB

4c2t Structural and Mechanistic Insight Into DNA Unwinding by Deinococcus Radiodurans Uvrd.

Chain

Resolution

3.997 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	K38 T39 D227 E228 Q258 R617
Catalytic site (residue number reindexed from 1)	K34 T35 D223 E224 Q254 R605
Enzyme Commision number	5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	F65 H93 R142 Y261 F263 R264 R362 T363 R422 G424 G426 T428 R459 L544 S546 T569 H571 N572 F633	F61 H89 R138 Y257 F259 R260 R358 T359 R418 G420 G422 T424 R455 L540 S542 T557 H559 N560 F621	PDBbind-CN: Kd=0.36uM
BS02	dna	A	R393 F633 G634	R389 F621 G622	PDBbind-CN: Kd=0.36uM
BS03	ANP	A	A12 Q17 G35 G37 K38 T39 R40 Q258 Y290 R291 R617	A8 Q13 G31 G33 K34 T35 R36 Q254 Y286 R287 R605

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003678	DNA helicase activity
GO:0004386	helicase activity
GO:0005524	ATP binding
GO:0016787	hydrolase activity
GO:0016853	isomerase activity
GO:0016887	ATP hydrolysis activity
GO:0043138	3'-5' DNA helicase activity
GO:0046872	metal ion binding

	Biological Process
GO:0000725	recombinational repair
GO:0032508	DNA duplex unwinding

	Cellular Component
GO:0005829	cytosol
GO:0033202	DNA helicase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:4c2t, PDBe:4c2t, PDBj:4c2t
PDBsum	4c2t
PubMed	24143224
UniProt	Q9RTI9

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