Structure of PDB 4bwm Chain A

Receptor sequence

>4bwmA (length=538) Species: 271 (Thermus aquaticus)

EEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRD
LKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRY
GGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHM
EATGVRLDVAYLRAMSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVL
FDELGLPAIGKTEKTGKRSTRAAVLEALREAHPIVEKILQYRELTKLKST
YIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRI
RRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDFHTETAS
WMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIE
RYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAA
ERKAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKE
RAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

3D structure

• PDB: 4bwm Structure and Function of an RNA-Reading Thermostable DNA Polymerase.
• Chain: A
• Resolution: 1.749 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R487 T506 K508 T509 S513 T514 R515 A516 Q582 N583 P585 V586 H784	R193 T212 K214 T215 S219 T220 R221 A222 Q288 N289 P291 V292 H490
BS02	rna	A	K508 K540 T569 A570 Q582 Y671 M673 S674 H676 R677 Y686 R728 S739 R746 K747 N750 Q754	K214 K246 T275 A276 Q288 Y377 M379 S380 H382 R383 Y392 R434 S445 R452 K453 N456 Q460
BS03	DCT	A	Q613 H639 R659 K663 F667	Q319 H345 R365 K369 F373

Gene Ontology

Molecular Function

• GO:0001882 nucleoside binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair

External links

• PDB: RCSB:4bwm, PDBe:4bwm, PDBj:4bwm
• PDBsum: 4bwm
• PubMed: 24106012
• UniProt: P19821|DPO1_THEAQ DNA polymerase I, thermostable (Gene Name=polA)