Structure of PDB 4buc Chain A

Receptor sequence

>4bucA (length=427) Species: 2336 (Thermotoga maritima)

PRGSHMKIGFLGFGKSNRSLLKYLLNHQEAKFFVSEAKTLDGETKKFLEE
HSVEYEEGGHTEKLLDCDVVYVSPGIKPDTSMIELLSSRGVKLSTELQFF
LDNVDPKKVVGITGTDGKSTATALMYHVLSGRGFKTFLGGNFGTPAVEAL
EGEYDYYVLEMSSFQLFWSERPYLSNFLVLNISEDHLDWHSSFKEYVDSK
LKPAFLQTEGDLFVYNKHIERLRNLEGVRSRKIPFWTDENFATEKELIVR
GKKYTLPGNYPYQMRENILAVSVLYMEMFNELESFLELLRDFKPLPHRME
YLGQIDGRHFYNDSKATSTHAVLGALSNFDKVVLIMCGIGKKENYSLFVE
KASPKLKHLIMFGEISKELAPFVGKIPHSIVENMEEAFEKAMEVSEKGDV
ILLSPGGASFAKRGEHFREIFKRHGGD

3D structure

• PDB: 4buc Mreb and Murg as Scaffolds for the Cytoplasmic Steps of Peptidoglycan Biosynthesis
• Chain: A
• Resolution: 2.17 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K133 N156 H201
• Catalytic site (residue number reindexed from 1): K118 N141 H186
• Enzyme Commision number: 6.3.2.9: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PO3	A	S96 M97	S81 M82

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008764 UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
• GO:0016874 ligase activity
• GO:0016881 acid-amino acid ligase activity

Biological Process

• GO:0008360 regulation of cell shape
• GO:0009058 biosynthetic process
• GO:0009252 peptidoglycan biosynthetic process
• GO:0051301 cell division
• GO:0071555 cell wall organization

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4buc, PDBe:4buc, PDBj:4buc
• PDBsum: 4buc
• PubMed: 23826965
• UniProt: Q9WY76|MURD_THEMA UDP-N-acetylmuramoylalanine--D-glutamate ligase (Gene Name=murD)