Structure of PDB 4beu Chain A

Receptor sequence

>4beuA (length=392) Species: 666 (Vibrio cholerae)

APLHIDTALPDAAQIQQSNSWLEISLGQFQSNIEQFKSHMNANTKICAIM
KADAYGNGIRGLMPTIIAQGIPCVGVASNAEARAVRESGFKGELIRVRSA
SLSEMSSALDLNIEELIGTHQQALDLAELAKQSGKTLKVHIALNDGGMGR
NGIDMTTEAGKKEAVSIATQPSLSVVGIMTHFPNYNADEVRAKLAQFKES
STWLMQQANLKREEITLHVANSYTALNVPEAQLDMVRPGGVLFGDLPTNP
EYPSIVSFKTRVSSLHHLPKDSTVGYDSTFTTSRDSVLANLPVGYSDGYP
RKMGNKAEVLINGQRAKVVGVTSMNTTVVDVTEIKGVLPGQEVVLFGQQQ
KQSIAVSEMENNAELIFPELYTLWGTSNPRFYVKSSGHHHHH

3D structure

• PDB: 4beu Structural Basis for the Broad Specificity of a New Family of Amino-Acid Racemases.
• Chain: A
• Resolution: 1.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K74 R173 H204 R260 Y299 S346 N348
• Catalytic site (residue number reindexed from 1): K51 R150 H181 R237 Y276 S323 N325
• Enzyme Commision number: 5.1.1.10: amino-acid racemase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	A	K74 Y78 R173 H204 N244 S245 R260 G262 G263	K51 Y55 R150 H181 N221 S222 R237 G239 G240

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0008784 alanine racemase activity
• GO:0016853 isomerase activity
• GO:0018111 methionine racemase activity
• GO:0018113 lysine racemase activity
• GO:0030170 pyridoxal phosphate binding
• GO:0030378 serine racemase activity
• GO:0047661 amino-acid racemase activity
• GO:0047679 arginine racemase activity
• GO:0050157 ornithine racemase activity

Biological Process

• GO:0006522 alanine metabolic process
• GO:0030632 D-alanine biosynthetic process

Cellular Component

• GO:0005829 cytosol
• GO:0042597 periplasmic space

External links

• PDB: RCSB:4beu, PDBe:4beu, PDBj:4beu
• PDBsum: 4beu
• PubMed: 24419381
• UniProt: Q9KSE5|BSR_VIBCH Broad specificity amino-acid racemase (Gene Name=bsrV)