Structure of PDB 4beb Chain A

Receptor sequence
>4bebA (length=839) Species: 562 (Escherichia coli) [Search protein sequence]
NFIVLDKYIKAEPTGDSYQSESDLERELIQDLRNQGYEFISVKSQSAMLA
NVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRKIHIDYICDFI
FDDERLENIYLIDKKNLMRNKVQIIQQFEQAGSHANRYDVTILVNGLPLV
QIELKKRGVAIREAFNQINSLFKYLQLFVISNGTDTRYFANTTKRDENSF
DFTMNWAKSDNTLIKDLKDFTATCFQKHTLLNVLVNYSVFDSSQTLLVMR
PYQIAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARL
ATELDFIDKVFFVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDK
DDNKIIVTTIQKLNNLMKAESDLPVYNQQVVFIFDECHRSQFGEAQKNLK
KKFKRYYQFGFTGTPIFPENALGSETTASVFGRELHSYVITDAIRDEKVL
KFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFLHPMRIQEITQYILNN
FRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAANKSATYKP
LRIATIFSFAEISDETFDTSAMDSSAKEFLDAAIREYNSHFKTNFSTDSN
GFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGL
MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKS
YTEYMEGFTDAATGEAKRGFMTVVSELEQRFPDPTSIESEKEKKDFVKLF
GEYLRAENILQNYDEFATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAEL
QTIRLPADRKIQDYRSAYNDIRDWQRRWDDVVFEVDLLK
3D structure
PDB4beb Functional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzyme.
ChainA
Resolution2.989 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.3: type I site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A T314 D408 T291 D385
BS02 ATP A M227 L270 V271 Q276 T309 G310 G312 K313 T314 L315 T661 D664 R691 M204 L247 V248 Q253 T286 G287 G289 K290 T291 L292 T629 D632 R659
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0005524 ATP binding
GO:0009035 type I site-specific deoxyribonuclease activity
Biological Process
GO:0006304 DNA modification
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:4beb, PDBe:4beb, PDBj:4beb
PDBsum4beb
PubMed26039067
UniProtP10486|T1R1_ECOLX Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit (Gene Name=hsdR)

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