Structure of PDB 4b8w Chain A

Receptor sequence

>4b8wA (length=319) Species: 9606 (Homo sapiens) [Search protein sequence]

NLYFQSMRILVTGGSGLVGKAIQKVVADGAGLPGEDWVFVSSKDADLTDT
AQTRALFEKVQPTHVIHLAAMVGGLFRNIKYNLDFWRKNVHMNDNVLHSA
FEVGARKVVSCLSTCIFPDKTTYPIDETMIHNGPPHNSNFGYSYAKRMID
VQNRAYFQQYGCTFTAVIPTNVFGPHDNFNIEDGHVLPGLIHKVHLAKSS
GSALTVWGTGNPRRQFIYSLDLAQLFIWVLREYNEVEPIILSVGEEDEVS
IKEAAEAVVEAMDFHGEVTFDTTKSDGQFKKTASNSKLRTYLPDFRFTPF
KQAVKETCAWFTDNYEQAR

3D structure

PDB

4b8w Crystal Structure of Human Gdp-L-Fucose Synthase with Bound Nadp and Gdp, Tetragonal Crystal Form

Chain

Resolution

2.75 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	S114 T115 C116 Y143 K147 H186
Catalytic site (residue number reindexed from 1)	S113 T114 C115 Y142 K146 H185
Enzyme Commision number	1.1.1.271: GDP-L-fucose synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAP	A	G14 S16 G17 L18 V19 S42 S43 D47 L48 L69 A70 A71 V73 M93 C112 Y143 K147 P170 T171 V173 H186 R320	G13 S15 G16 L17 V18 S41 S42 D46 L47 L68 A69 A70 V72 M92 C111 Y142 K146 P169 T170 V172 H185 R319
BS02	GDP	A	G75 L76 F77 N172 H186 V187 W208 R215 D277 K282	G74 L75 F76 N171 H185 V186 W207 R214 D276 K281

Gene Ontology

	Molecular Function
GO:0005515	protein binding
GO:0009055	electron transfer activity
GO:0016491	oxidoreductase activity
GO:0016853	isomerase activity
GO:0042356	GDP-4-dehydro-D-rhamnose reductase activity
GO:0042802	identical protein binding
GO:0047918	GDP-mannose 3,5-epimerase activity
GO:0050577	GDP-L-fucose synthase activity

	Biological Process
GO:0007159	leukocyte cell-cell adhesion
GO:0009226	nucleotide-sugar biosynthetic process
GO:0010595	positive regulation of endothelial cell migration
GO:0019673	GDP-mannose metabolic process
GO:0042351	'de novo' GDP-L-fucose biosynthetic process
GO:1904906	positive regulation of endothelial cell-matrix adhesion via fibronectin

	Cellular Component
GO:0005829	cytosol
GO:0070062	extracellular exosome

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:4b8w, PDBe:4b8w, PDBj:4b8w
PDBsum	4b8w
PubMed
UniProt	Q13630\|FCL_HUMAN GDP-L-fucose synthase (Gene Name=GFUS)

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