Structure of PDB 4b6f Chain A

Receptor sequence

>4b6fA (length=645) Species: 11105 (Hepatitis C virus (isolate BK))

ITAYSQQTRGLLGCIITSLTGRDKNQVEGEVQVVSTATQSFLATCVNGVC
WTVYHGAGSKTLAGPKGPITQMYTNVDQDLVGWQAPPGARSLTPCTCGSS
DLYLVTRHADVIPVRRRGDSRGSLLSPRPVSYLKGSSGGPLLCPSGHAVG
IFRAAVCTRGVAKAVDFVPVESMETTMRSPVFTDNSSPPAVPQSFQVAHL
HAPTGSGKSTKVPAAYAAQGYKVLVLNPSVAATLGFGAYMSKAHGIDPNI
RTGVRTITTGAPVTYSTYGKFLADGGCSGGAYDIIICDECHSTDSTTILG
IGTVLDQAETAGARLVVLATATPPGSVTVPHPNIEEVALSNTGEIPFYGK
AIPIEAIRGGRHLIFCHSKKKCDELAAKLSGLGINAVAYYRGLDVSVIPT
IGDVVVVATDALMTGYTGDFDSVIDCNTCVTQTVDFSLDPTFTIETTTVP
QDAVSRSQRRGRTGRGRRGIYRFVTPGERPSGMFDSSVLCECYDAGCAWY
ELTPAETSVRLRAYLNTPGLPVCQDHLEFWESVFTGLTHIDAHFLSQTKQ
AGDNFPYLVAYQATVCARAQAPPPSWDQMWKCLIRLKPTLHGPTPLLYRL
GAVQNEVTLTHPITKYIMACMSADLEVVTGSVVIVGRIILSGSGS

3D structure

• PDB: 4b6f Discovery of an Allosteric Mechanism for the Regulation of Hcv Ns3 Protein Function.
• Chain: A
• Resolution: 2.89 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T10 C16 H57 D81 G137 S139
• Catalytic site (residue number reindexed from 1): T8 C14 H55 D79 G135 S137
• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.98: hepacivirin.
  3.4.22.-
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	20L	A	D79 D81 R155 M485 V524 Q526 V630	D77 D79 R153 M483 V522 Q524 V628	PDBbind-CN: -logKd/Ki=3.30,IC50~500uM

Gene Ontology

Molecular Function

• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0008236 serine-type peptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019087 transformation of host cell by virus

External links

• PDB: RCSB:4b6f, PDBe:4b6f, PDBj:4b6f
• PDBsum: 4b6f
• PubMed: 23023261
• UniProt: P26663|POLG_HCVBK Genome polyprotein