Structure of PDB 4azw Chain A

Receptor sequence

>4azwA (length=413) Species: 562 (Escherichia coli)

LNTLVSELPEIYQTIFGHPEWDGDAARDCNQRLDLITEQYDNLSRALGRP
LNVLDLGCAQGFFSLSLASKGATIVGIDFQQENINVCRALAEENPDFAAE
FRVGRIEEVIAALEEGEFDLAIGLSVFHHIVHLHGIDEVKRLLSRLADVT
QAVILELAVKEEPFYWGVSQPDDPRELIEQCAFYRLIGEFDRPMYLVSNH
RVLINDFNQPFQHWQNQPYAGAGLAHKRSRRYFFGEDYVCKFFYYDMPHG
ILTAEESQRNKHELHNEIKFLTQPPAGFDAPAVLAHGENAQSGWLVMEKL
PGRLLSDMLAAGEEIDREKILGSLLRSLAALEKKGFWHDDVRPWNVMVDA
RQHARLIDFGSIVTTPTNLVQSFFVFVNELFLPQPWSNWLYAVWQEPVER
WNFALLLALFEKK

3D structure

• PDB: 4azw Structure of Wbdd; a Bifunctional Kinase and Methyltransferase that Regulates the Chain Length of the O Antigen in Escherichia Coli O9A.
• Chain: A
• Resolution: 2.47 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.294: 3-O-phospho-polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol 3-phospho-methyltransferase.
  2.7.1.181: polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	P229 H237 R241 Y243 K252 M308 K310 L311 I368 D369	P218 H226 R230 Y232 K241 M297 K299 L300 I357 D358
BS02	SAM	A	Y16 Q17 R36 G61 A63 D82 F83 Q84 R109 I110 E111 L128 S129 V130	Y12 Q13 R32 G57 A59 D78 F79 Q80 R105 I106 E107 L124 S125 V126
BS03	MG	A	N356 D369	N345 D358
BS04	MG	A	E274 D369	E263 D358

Gene Ontology

Molecular Function

• GO:0004672 protein kinase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006468 protein phosphorylation

External links

• PDB: RCSB:4azw, PDBe:4azw, PDBj:4azw
• PDBsum: 4azw
• PubMed: 22970759
• UniProt: Q47592|WBDD2_ECOLX O-antigen chain terminator bifunctional methyltransferase/kinase WbdD (Gene Name=wbdD)