Structure of PDB 4ayr Chain A

Receptor sequence
>4ayrA (length=432) Species: 366602 (Caulobacter sp. K31) [Search protein sequence]
EDWKALAADVRSEFQWAWQGYVAKAWGKDEINPVSGTSRSFFIEGHDLGL
SLVEALDTLWIMGLDAEFQAGVDWVKANLSFDVDGNAQVFETNIRLVGGL
LSAHLASGDPVLLAKARDLADRLAKAFEASPHGLPWRYVNLRTGAVSDPE
TNLAEIGTYLSEFGVLSQLTGERKYFDMAKRAMRHTLDRRSKIGLMAANI
HAMTGAFTSRNASIDVYADSFYEYLWDAWALFGDEDCKRWAVECVDAQLA
HQAKRYDGRLWFPMVDFETGAVTGTAQSELAAYYAGLLGQVGRKAQGDDY
LASFTYLQATFGVIPESIDVTTGQPRRKHTGLRPEYPDACLNLWLIDRDP
RYRRLAAIHYREMKATSRAAFGYTALKDITTRPMTQDDNCPGYWWSEQMK
YYYLLFSDTPRIDYGQLQLSTEANVLRGFRKV
3D structure
PDB4ayr The Reaction Coordinate of a Bacterial Gh47 Alpha-Mannosidase: A Combined Quantum Mechanical and Structural Approach.
ChainA
Resolution1.1 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E121 R125 D249 E365
Catalytic site (residue number reindexed from 1) E91 R95 D219 E335
Enzyme Commision number 3.2.1.113: mannosyl-oligosaccharide 1,2-alpha-mannosidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 IFL A R363 P364 E365 Y423 E427 T451 E452 R333 P334 E335 Y393 E397 T421 E422 MOAD: Kd=99nM
PDBbind-CN: -logKd/Ki=7.00,Kd=99nM
BS02 IFL A F120 E185 Y247 D249 F90 E155 Y217 D219 MOAD: Kd=99nM
PDBbind-CN: -logKd/Ki=7.00,Kd=99nM
Gene Ontology
Molecular Function
GO:0004559 alpha-mannosidase activity
GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
GO:0005509 calcium ion binding
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0046872 metal ion binding
Biological Process
GO:0005975 carbohydrate metabolic process
GO:1904382 mannose trimming involved in glycoprotein ERAD pathway
Cellular Component
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4ayr, PDBe:4ayr, PDBj:4ayr
PDBsum4ayr
PubMed23012075
UniProtB0SWV2

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