Structure of PDB 4aq7 Chain A

Receptor sequence

>4aq7A (length=860) Species: 562 (Escherichia coli) [Search protein sequence]

MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHM
GHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPW
TYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVY
KKTSAVNWCPNDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYAD
ELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYDNTLTVYTTRP
DTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATMEK
KGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFAS
KYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNAIA
DKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYY
ARYTCPQYKEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRD
AGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGR
IVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFAS
PADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTENQKA
LRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAPTDGEQDRALM
QEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDST
LVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYV
PGKLLNLVVG

3D structure

PDB

4aq7 Structural Dynamics of the Aminoacylation and Proofreading Functional Cycle of Bacterial Leucyl-tRNA Synthetase

Chain

Resolution

2.5 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	L41 H49 H52 D80 C159 D162 C176 C179 F493 H533 F543 K619 K622
Catalytic site (residue number reindexed from 1)	L41 H49 H52 D80 C159 D162 C176 C179 F493 H533 F543 K619 K622
Enzyme Commision number	6.1.1.4: leucine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	A	Y43 D80 G83 L84 V156 A167 N168 E169 M219 N222 W223 A291 E292 R416 L417 R418 R424 Q425 F493 E532 H533 M617 S618 K619 F648 A649 S650 P651 M654 Q659 S661 G662 G665 R668 K711 R719 N723 M730 E731 N734 V803 R811 K813 K837 Y838 I848 V850 N856 V858	Y43 D80 G83 L84 V156 A167 N168 E169 M219 N222 W223 A291 E292 R416 L417 R418 R424 Q425 F493 E532 H533 M617 S618 K619 F648 A649 S650 P651 M654 Q659 S661 G662 G665 R668 K711 R719 N723 M730 E731 N734 V803 R811 K813 K837 Y838 I848 V850 N856 V858
BS02	LMS	A	M40 P42 Y43 G51 H52 N55 G530 E532 Q566 M568 V569 M620	M40 P42 Y43 G51 H52 N55 G530 E532 Q566 M568 V569 M620
BS03	LEU	A	L41 D80 S496 H533 H537	L41 D80 S496 H533 H537

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0002161	aminoacyl-tRNA editing activity
GO:0004812	aminoacyl-tRNA ligase activity
GO:0004823	leucine-tRNA ligase activity
GO:0005515	protein binding
GO:0005524	ATP binding

	Biological Process
GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation
GO:0006429	leucyl-tRNA aminoacylation
GO:0106074	aminoacyl-tRNA metabolism involved in translational fidelity

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol

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Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:4aq7, PDBe:4aq7, PDBj:4aq7
PDBsum	4aq7
PubMed	22683997
UniProt	P07813\|SYL_ECOLI Leucine--tRNA ligase (Gene Name=leuS)

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