Structure of PDB 4afk Chain A

Receptor sequence

>4afkA (length=430) Species: 208964 (Pseudomonas aeruginosa PAO1)

EAPKNFGLDVKITGESENDRDLGTAPGGTLNDIGIDLRPWAFGQWGDWSA
YFMGQAVAATDTIETDTLQSDTDGREPDKSYLAAREFWVDYAGLTAYPGE
HLRFGRQRLREDSGQWQDTNIEALNWSFETTLLNAHAGVAQRFSEYRTDL
DELAPEDKDRTHVFGDISTQWAPHHRIGVRIHHADDSGHLRRPGEEVDNL
DKTYTGQLTWLGIEATGDAYNYRSSMPLNYWASATWLTGDRDNLTTTTVD
DRRIATGKQSGDVNAFGVDLGLRWNIDEQWKAGVGYARGSGGGKDGEEQF
QQTGLESNRSNFTGTRSRVHRFGEAFRGELSNLQAATLFGSWQLREDYDA
SLVYHKFWRVDDDSDIGTSGINAALQPGEKDIGQELDLVVTKYFSALIRF
RGGLFKPGDAYGPGTDSTMHRAFVDFIWRF

3D structure

• PDB: 4afk A Conformational Landscape for Alginate Secretion Across the Outer Membrane of Pseudomonas Aeruginosa.
• Chain: A
• Resolution: 1.897 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D55 D57 A61 P62 G63	D19 D21 A25 P26 G27
BS02	FLC	A	R74 D102 T103 R152	R38 D66 T67 R108
BS03	78M	A	W81 Y87 F88 F148	W45 Y51 F52 F104
BS04	78N	A	S188 Y190	S144 Y146
BS05	78M	A	W170 V207 F208 I225 H227	W126 V163 F164 I181 H183
BS06	78N	A	W215 H219	W171 H175
BS07	78N	A	Y392 V434 I458	Y348 V390 I398
BS08	78M	A	N54 L430 G463 L464 T478 H480	N18 L386 G403 L404 T418 H420

Gene Ontology

Biological Process

• GO:0042121 alginic acid biosynthetic process

Cellular Component

• GO:0009279 cell outer membrane

External links

• PDB: RCSB:4afk, PDBe:4afk, PDBj:4afk
• PDBsum: 4afk
• PubMed: 25084326
• UniProt: P18895|ALGE_PSEAE Alginate production protein AlgE (Gene Name=algE)