Structure of PDB 4a0r Chain A

Receptor sequence
>4a0rA (length=755) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]
PFHLPLNHPTYLIWSANTSLGKTLVSTGIAASFLLQQPSSSATKLLYLKP
IQTGFPSDSDSRFVFSKLDSLSLRRQIPISISNSVLHSSLPAAKSLGLNV
EVSESGMCSLNFRDEKTVTGAPELLCKTLYAWEAAISPHLAAERENATVE
DSVVLQMIEKCLKEEMDLLCLVETAGGVASPGPSGTLQCDLYRPFRLPGI
LVGDGRLGGISGTIAAYESLKLRGYDIAAVVFEDHGLVNEVPLTSYLRNK
VPVLVLPPVPKDPSDDLIEWFVESDGVFKALKETMVLANLERLERLNGMA
KLAGEVFWWPFTQHKLVHQETVTVIDSRCGENFSIYKASDNSSLSQQFDA
CASWWTQGPDPTFQAELAREMGYTAARFGHVMFPENVYEPALKCAELLLD
GVGKGWASRVYFSDNGSTAIEIALKMAFRKFCVDHNFIVVKVIALRGSYH
GDTLGAMEAQAPSPYTGFLQQPWYTGRGLFLDPPTVFLSNGSWNISLTFT
SRDEIFDKSRDASTLARIYSAYLSKHLAHVGALIIEPVIHGAGGMHMVDP
LFQRVLVNECRNRKIPVIFDEVFTGFWRLGVETTTELLGCKPDIACFAKL
LTGGMVPLAVTLATDAVFDSFSGDSKLKALLHGHSYSAHAMGCATAAKAI
QWFKDPETNHNITSQGKTLRELWDEELVQQISSHSAVQRVVVIGTLFALE
LKSLYAKSLLIMLREDGIFTRPLGNVIYLMCGPCTSPEICRRLLTKLYKR
LGEFN
3D structure
PDB4a0r Biochemical and Structural Characterization of the Arabidopsis Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis.
ChainA
Resolution2.68 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) F326 Y473 D615 K644
Catalytic site (residue number reindexed from 1) F311 Y449 D570 K599
Enzyme Commision number 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.
6.3.3.3: dethiobiotin synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PLP A N430 G431 S432 Y473 H474 E581 D615 V617 K644 N415 G416 S417 Y449 H450 E536 D570 V572 K599
BS02 DTB A T24 Q58 T59 S143 S195 T18 Q52 T53 S137 S180
BS03 PLP A H679 S680 H634 S635
BS04 DTB A G223 I225 G208 I210
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0004141 dethiobiotin synthase activity
GO:0005524 ATP binding
GO:0008483 transaminase activity
GO:0016874 ligase activity
GO:0030170 pyridoxal phosphate binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0009102 biotin biosynthetic process
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:4a0r, PDBe:4a0r, PDBj:4a0r
PDBsum4a0r
PubMed22547782
UniProtB0F481|BIODA_ARATH Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial (Gene Name=BIO3-BIO1)

[Back to BioLiP]