Structure of PDB 3zwa Chain A

Receptor sequence
>3zwaA (length=727) Species: 10116 (Rattus norvegicus) [Search protein sequence]
VPRGSHMAEYLRLPHSLAMIRLCNPPVNAVSPTVIREVRNGLQKAGSDHT
VKAIVICGANGNFCAGADIHGFSAFTPGLALGSLVDEIQRYQKPVLAAIQ
GVALGGGLELALGCHYRIANAKARVGLPEVTLGILPGARGTQLLPRVVGV
PVALDLITSGKYLSADEALRLGILDAVVKSDPVEEAIKFAQKIIDKPIEP
RRIFNKPVPSLPNMDSVFAEAIAKVRKQYPGVLAPETCVRSIQASVKHPY
EVGIKEEEKLFMYLRASGQAKALQYAFFAEKSANKWSTPSGASWKTASAQ
PVSSVGVLGLGTMGRGIAISFARVGISVVAVESDPKQLDAAKKIITFTLE
KEASRAHQNGQASAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAE
LSALCKPGAFLCTNTSALNVDDIASSTDRPQLVIGTHFFSPAHVMRLLEV
IPSRYSSPTTIATVMSLSKKIGKIGVVVGNCYGFVGNRMLAPYYNQGFFL
LEEGSKPEDVDGVLEEFGFKMGPFRVSDLAGLDVGWKIRKGQGLTGPSLP
PGTPVRKRGNSRYSPLGDMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDPW
LSTFLSQYREVHHIEQRTISKEEILERCLYSLINEAFRILEEGMAARPEH
IDVIYLHGYGWPRHKGGPMFYAASVGLPTVLEKLQKYYRQNPDIPQLEPS
DYLRRLVAQGSPPLKEWQSLAGPHGSK
3D structure
PDB3zwa The Isomerase and Hydratase Reaction Mechanism of the Crotonase Active Site of the Multifunctional Enzyme (Type-1), as Deduced from Structures of Complexes with 3S-Hydroxy- Acyl-Coa.
ChainA
Resolution2.47 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) A61 F66 G76 G100 E103 P122 E123 P130 G131 K249 S410 H431 E443 N481
Catalytic site (residue number reindexed from 1) A67 F72 G82 G106 E109 P128 E129 P136 G137 K255 S416 H437 E449 N487
Enzyme Commision number 1.1.1.35: 3-hydroxyacyl-CoA dehydrogenase.
4.2.1.17: enoyl-CoA hydratase.
5.3.3.8: Delta(3)-Delta(2)-enoyl-CoA isomerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 3H9 A V21 A59 G60 A61 D62 I63 L98 E103 R118 E123 L126 G131 Y156 V27 A65 G66 A67 D68 I69 L104 E109 R124 E129 L132 G137 Y162
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity
GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity
GO:0004300 enoyl-CoA hydratase activity
GO:0016491 oxidoreductase activity
GO:0016509 long-chain-3-hydroxyacyl-CoA dehydrogenase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0016829 lyase activity
GO:0016853 isomerase activity
GO:0016863 intramolecular oxidoreductase activity, transposing C=C bonds
GO:0019899 enzyme binding
GO:0070403 NAD+ binding
Biological Process
GO:0006631 fatty acid metabolic process
GO:0006635 fatty acid beta-oxidation
Cellular Component
GO:0005777 peroxisome
GO:0005829 cytosol

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Molecular Function
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Cellular Component
External links
PDB RCSB:3zwa, PDBe:3zwa, PDBj:3zwa
PDBsum3zwa
PubMed23351063
UniProtP07896|ECHP_RAT Peroxisomal bifunctional enzyme (Gene Name=Ehhadh)

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