Structure of PDB 3zjv Chain A

Receptor sequence

>3zjvA (length=813) Species: 562 (Escherichia coli)

MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHM
GHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPW
TYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVY
KKTSAVEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEGV
EITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAA
FIDECRNTMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVP
GHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNG
LDHEAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLED
GTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDT
FDTFMESSWYYARYTCPQYKEGMLDSEAANYWLPVDIYIGGIEHAIMHLL
YFRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPV
DAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGA
DTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAAL
NVDALTENQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKA
PTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPV
ADEKAMVEDSTLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYV
IYVPGKLLNLVVG

3D structure

• PDB: 3zjv Discovery of a Novel Class of Boron-Based Antibacterials with Activity Against Gram-Negative Bacteria.
• Chain: A
• Resolution: 2.31 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L41 H49 H52 D80 F493 H533 F543 K619 K622
• Catalytic site (residue number reindexed from 1): L41 H49 H52 D80 F454 H494 F504 K580 K583
• Enzyme Commision number: 6.1.1.4: leucine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S227 Y246 T247 T248 R249 M298 E299 K300 F325 V326 L327 Y330 V335 M336 D342 R344 D345 R416 R418 K598 R600 F648 E657 S661 R668 R672 K711 R719 N723 A727 M730 E731 N734 K813 I848 V850	S197 Y216 T217 T218 R219 M259 E260 K261 F286 V287 L288 Y291 V296 M297 D303 R305 D306 R377 R379 K559 R561 F609 E618 S622 R629 R633 K672 R680 N684 A688 M691 E692 N695 K774 I801 V803

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0002161 aminoacyl-tRNA editing activity
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004823 leucine-tRNA ligase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding

Biological Process

• GO:0006412 translation
• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006429 leucyl-tRNA aminoacylation
• GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3zjv, PDBe:3zjv, PDBj:3zjv
• PDBsum: 3zjv
• PubMed: 23295920
• UniProt: P07813|SYL_ECOLI Leucine--tRNA ligase (Gene Name=leuS)