Structure of PDB 3zj6 Chain A

Receptor sequence
>3zj6A (length=469) Species: 4060 (Rauvolfia serpentina) [Search protein sequence]
DATRISRSDFPADFIMGTGSSAYQIEGGARDGGRGPSIWDTFTHRRPDMI
RGGTNGDVAVDSYHLYKEDVNILKNLGLDAYRFSISWSRVLPGGRLSGGV
NKEGINYYNNLIDGLLANGIKPFVTLFHWDVPQALEDEYGGFLSPRIVDD
FCEYAELCFWEFGDRVKHWMTLNEPWTFSVHGYATGLYAPGRGRTGNPGT
EPYWVTHHLLLAHAAAVELYKNKFQRGQEGQIGISHATQWMEPWDENSAS
DVEAAARALDFMLGWFMEPITSGDYPKSMKKFVGSRLPKFSPEQSKMLKG
SYDFVGLNYYTASYVTNASNFSYNTDIHVTYETDRNGVPIGPQSGSDWLL
IYPEGIRKILVYTKKTYNVPLIYVTENGVDDVKNTNLTLSEARKDSMRLK
YLQDHIFNVRQAMNDGVNVKGYFAWSLLDNFEWGEGYGVRFGIIHIDYND
NFARYPKDSAVWLMNSFHK
3D structure
PDB3zj6 Ligand Structures of Synthetic Deoxa-Pyranosylamines with Raucaffricine and Strictosidine Glucosidases Provide Structural Insights Into Their Binding and Inhibitory Behaviours.
ChainA
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R94 H140 E186 T189 N345 Y347 E420
Catalytic site (residue number reindexed from 1) R82 H128 E174 T177 N308 Y310 E376
Enzyme Commision number 2.4.1.219: vomilenine glucosyltransferase.
3.2.1.125: raucaffricine beta-glucosidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 VM2 A Q36 H140 E186 T189 H193 Y347 W392 E420 W469 E476 W477 Q24 H128 E174 T177 H181 Y310 W348 E376 W425 E432 W433 PDBbind-CN: -logKd/Ki=6.66,Ki=0.22uM
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0008422 beta-glucosidase activity
GO:0016740 transferase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0050247 raucaffricine beta-glucosidase activity
GO:0050506 vomilenine glucosyltransferase activity
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0009820 alkaloid metabolic process
GO:0009821 alkaloid biosynthetic process

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Molecular Function
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Biological Process
External links
PDB RCSB:3zj6, PDBe:3zj6, PDBj:3zj6
PDBsum3zj6
PubMed25140865
UniProtQ9SPP9|RG1_RAUSE Raucaffricine-O-beta-D-glucosidase (Gene Name=RG)

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