Structure of PDB 3xim Chain A

Receptor sequence

>3ximA (length=391) Species: 1866 (Actinoplanes missouriensis)

QATREDKFSFGLWTVGWQARDAFGDATRTALDPVEAVHKLAEIGAYGITF
HDDDLVPFGSDAQTRDGIIAGFKKALDETGLIVPMVTTNLFTHPVFKDGG
FTSNDRSVRRYAIRKVLRQMDLGAELGAKTLVLWGGREGAEYDSAKDVSA
ALDRYREALNLLAQYSEDRGYGLRFAIEPKPNEPRGDILLPTAGHAIAFV
QELERPELFGINPETGHEQMSNLNFTQGIAQALWHKKLFHIDLNGQHGPK
FDQDLVFGHGDLLNAFSLVDLLENGPDGAPAYDGPRHFDYKPSRTEDYDG
VWESARANIRMYLLLRERARAFRADPEVQEALAASKVAELKTPTLNPGEG
YAELLADRSAFEDYDADAVGAKGFGFVKLNQLAIEHLLGAR

3D structure

• PDB: 3xim Arginine residues as stabilizing elements in proteins.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H54 D57 M88 E181 K183 E217 H220 D245 D255 D257 D292
• Catalytic site (residue number reindexed from 1): H51 D54 M85 E178 K180 E214 H217 D242 D252 D254 D289
• Enzyme Commision number: 5.3.1.5: xylose isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SOR	A	H54 T90 V135 W137 E181 E217 H220 D292	H51 T87 V132 W134 E178 E214 H217 D289
BS02	CO	A	E181 E217 D245 D292	E178 E214 D242 D289
BS03	CO	A	E217 H220 D255	E214 H217 D252

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0009045 xylose isomerase activity
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0042732 D-xylose metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3xim, PDBe:3xim, PDBj:3xim
• PDBsum: 3xim
• PubMed: 1540579
• UniProt: P12851|XYLA_ACTM4 Xylose isomerase (Gene Name=xylA)