Structure of PDB 3wye Chain A

Receptor sequence
>3wyeA (length=255) Species: 573,1718 [Search protein sequence]
KKVALVTGAGQGIGKAIALRLVKDGFAVAIADYNDATAKAVASEINQAGG
HAVAVKVDVSDRDQVFAAVEQARKTLGGFDVIVNNAGIAQIKPLLEVTEE
DLKQIYSVNVFSVFFGIQAAVEAFKKEGHGGKIINAASIAAIQGFPILSA
YSTTKFAVRGLTQTAARDLAPLGITVNGYCPGIVGTGMWEQIDAELSKIN
GKPIGENFKEYSSSIALGRPSVPEDVAGLVSFLASPDSDYMTGQSLLIDG
GMVFN
3D structure
PDB3wye Crystal Structure of chimeric engineered (2S,3S)-butanediol dehydrogenase complexed with NAD+
ChainA
Resolution1.58 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G13 S139 Y152 K156 L197
Catalytic site (residue number reindexed from 1) G12 S138 Y151 K155 L196
Enzyme Commision number 1.1.1.304: diacetyl reductase [(S)-acetoin forming].
1.1.1.76: (S,S)-butanediol dehydrogenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAD A G9 Q12 G13 I14 D33 Y34 V58 D59 V60 N86 A87 S139 Y152 K156 P182 G183 V185 T187 M189 W190 G8 Q11 G12 I13 D32 Y33 V57 D58 V59 N85 A86 S138 Y151 K155 P181 G182 V184 T186 M188 W189
Gene Ontology
Molecular Function
GO:0016491 oxidoreductase activity
GO:0019152 acetoin dehydrogenase (NAD+) activity
Biological Process
GO:0045150 acetoin catabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:3wye, PDBe:3wye, PDBj:3wye
PDBsum3wye
PubMed
UniProtQ48436|BUDC_KLEPN Diacetyl reductase [(S)-acetoin forming] (Gene Name=budC);
Q9ZNN8|BUDC_CORGT L-2,3-butanediol dehydrogenase (Gene Name=budC)

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