Structure of PDB 3wqy Chain A

Receptor sequence

>3wqyA (length=905) Species: 224325 (Archaeoglobus fulgidus DSM 4304)

TLDEEYLDITFLTENGFVRKRCPKCGKHFWTADPEREICGDPPCESYSFI
GNPVFKKPFELDEMREYYLNFFERRGHGRIERYPVVARWRTDIYLTIASI
ADFQPFVTSGVAPPPANPLTISQPCIRLDDLDSVGRTGRHLTLFEMMAHH
AFNYPGKEIYWKNETVAYCTELLNELGVKKEDIVYKEEPWAGGGNAGPCL
EAIVGGLEVATLVFMNLEEHPEGDIEIKGARYRKMDNYIVDTGYGLERFV
WASKGTPTVYDAIFPEVVDTIIDNSNVSFNREDERVRRIVAESSKLAGIM
GELRGERLNQLRKSVADTVGVSVEELEGIVVPLEKVYSLADHTRCILFML
GDGLVPSNAGAGYLARLMIRRSLRLAEELELGLDLYDLVEMHKKILGFEF
DVPLSTVQEILELEKERYRTTVSKGTRLVERLVERKKKLEKDDLIELYDS
HGIPVELAVGIAAEKGAEVEMPKDIYAELAKRHSKAEKVQEKKITLQNEY
PATEKLYYDDPTLLEFEAEVIGVEGDFVILNRSAFYPESGGQDNDVGYLI
ANGGKFEVVDVLEADGVVLHVVKGAKPEVGTKVKGVIDSDVRWRHMRHHS
ATHVLLYSLQKVLGNHVWQAGARKEFSKARLDVTHFRRPSEEEIKEIEML
ANREILANKPIKWEWMDRIEAERKFGFRLYQGGVPPGRKIRVVQVGDDVQ
ACGGTHCRSTGEIGMLKILKVESIQDGVIRFEFAAGEAAIEAVEEMERLL
REASSILRVEPAKLPKTVERFFEEWKDQRKEIERLKSVIADLWADILMER
AEEFDSMKVVAEVVDADMQALQKLAERLAEKGAVGCLMAKGEGKVFVVTF
SGQKYDARELLREIGRVAKGSGGGRKDVAQGAVQQLLDREEMLDVIFRFL
SEHEG

3D structure

• PDB: 3wqy The selective tRNA aminoacylation mechanism based on a single G.U pair
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.7: alanine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Y95 R128 E189 P190 W191 N359 A360 G361 Y364 T422 R428 R432 R436 E447 Y449 D450 S451 H452 G453 K781 G873 G874 G875 R876 D878 A883	Y94 R127 E188 P189 W190 N358 A359 G360 Y363 T421 R427 R431 R435 E446 Y448 D449 S450 H451 G452 K780 G872 G873 G874 R875 D877 A882
BS02	A5A	A	R128 H141 L142 F145 M147 W191 E209 A211 T212 D242 G244 G246 R249	R127 H140 L141 F144 M146 W190 E208 A210 T211 D241 G243 G245 R248

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0000166 nucleotide binding
• GO:0002161 aminoacyl-tRNA editing activity
• GO:0003676 nucleic acid binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004813 alanine-tRNA ligase activity
• GO:0005524 ATP binding
• GO:0008270 zinc ion binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0006419 alanyl-tRNA aminoacylation
• GO:0043039 tRNA aminoacylation
• GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3wqy, PDBe:3wqy, PDBj:3wqy
• PDBsum: 3wqy
• PubMed: 24919148
• UniProt: O28029|SYA_ARCFU Alanine--tRNA ligase (Gene Name=alaS)