Structure of PDB 3wnm Chain A

Receptor sequence

>3wnmA (length=701) Species: 1397 (Niallia circulans)

SGGIERVFTDKARYNPGDAVSIRVQAKNGTGSSWSGAARLEIFHLENSVY
TSSQSLSLTNGQSTTLTFTWTAPSTDFRGYFVRIDAGTLGQGATAIDVSS
DFTKYPRYGYISEFESGETALESKAKVDQLAQDYHINAWQFYDWMWRHDK
MIKRTGGSIDSTWLDLFNREISWSTLQNQIDAVHDVNGKAMAYAMIYASR
ENYSPLGISPTWGIYEDSSHTNQFDVDFGDGSTYLYMFDPQNPNWQNYIH
AEYIDSINTAGFDGIHVAQMGQRSNVYDYNGNSIDLSTRFSPFLDQAKSV
LSANNPARDNLTYNIVDGTVNGWAVNDVSKNADLDFLYSEIWYLSDSYNQ
LKNYIEQLRANGGNKAVVLAAYMNYADNAGTRYEAESASMTNVSTNTNHA
GYTGSGFVDQFASTGDKVSFAINAPEAGDYSLVFRYGNNTGANSTLNLYV
DGNFVQKLYFFNQSSWGTWKHDAWYQVPLTQGAHTVELRYESGNVGAVNL
DSLTLGTFDEHSVRLADAMMSASGATHIELGDDNQMLPHEYYPNRSKTMR
SSLKNAMKDHYNFITAYENLLFDSDVVPNDTGSQFVNLTGVSASGDGSAN
TVWYINKRTSDYNIVHLINLLGNDNQWRNTASQPSFQTNLPAKIYIGADE
TISDVYLASPDLSGGETQELAFTSGTDAGGKYVSFTVPELKYWNMIYMLE
H

3D structure

• PDB: 3wnm Structural elucidation of the cyclization mechanism of alpha-1,6-glucan by Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase.
• Chain: A
• Resolution: 2.25 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.248: cycloisomaltooligosaccharide glucanotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	A	Y182 Y233 Q309 N354 E380 E580	Y142 Y193 Q269 N314 E340 E540
BS02	GLC	A	Y182 D183 H188 F207 Y233 Y581	Y142 D143 H148 F167 Y193 Y541
BS03	GLC	A	F268 E580	F228 E540
BS04	GLC	A	Q312 D357	Q272 D317
BS05	GLC	A	E380 W382	E340 W342
BS06	GLC	A	H439 Q450 W506 N539	H399 Q410 W466 N499
BS07	CA	A	E424 E426 T443 G446 D541	E384 E386 T403 G406 D501

Gene Ontology

Molecular Function

• GO:0030246 carbohydrate binding

External links

• PDB: RCSB:3wnm, PDBe:3wnm, PDBj:3wnm
• PDBsum: 3wnm
• PubMed: 24616103
• UniProt: P94286|CTA1_NIACI Cycloisomaltooligosaccharide glucanotransferase