Structure of PDB 3wms Chain A

Receptor sequence
>3wmsA (length=685) Species: 44252 (Paenibacillus macerans) [Search protein sequence]
SPDTSVDNKVNFSTDVIYQIVTDRFADGDRTNNPAGDAFSGDRSNLKLYF
GGDWQGIIDKINDGYLTGMGVTALWISQPVENITSVIKYSGVNNTSYHGY
WARDFKQTNDAFGDFADFQNLIDTAHAHNIKVVIDFAPNHTSPADRDNPG
FAENGALYDNGSLLGAYSNDTAGLFHHNGGTDFSTIEDGIYKNLIDLADI
NHNNNAMDAYFKSAIDLWLGMGVDGIRFDAVKHMPFGWQKSFVSSIYGGD
HPVFTFGEWYLGADQTDGDNIKFANESGMNLLDFEYAQEVREVFRDKTET
MKDLYEVLASTESQYDYINNMVTFIDNHDMDRFQVAGSGTRATEQALALT
LTSRGVPAIYYGTEQYMTGDGDPNNRAMMTSFNTGTTAYKVIQALAPLRK
SNPAIAYGTTTERWVNNDVLIIERKFGSSAALVAINRNSSAAYPISGLLS
SLPAGTYSDVLNGLLNGNSITVGSGGAVTNFTLAAGGTAVWQYTAPETSP
AIGNVGPTMGQPGNIVTIDGRGFGGTAGTVYFGTTAVTGSGIVSWEDTQI
KAVIPKVAAGKTGVSVKTSSGTASNTFKSFNVLTGDQVTMRFLVNQANTN
YGTNVYLVGNAAELGSWDPNKAIGPMYNQVIAKYPSWYYDVSVPAGTKLD
FKFIKKGGGTVTWEGGGNHTYTTPASSVGTVTVDW
3D structure
PDB3wms Structural basis of a mutant Y195I alpha-cyclodextrin glycosyltransferase with switched product specificity from alpha-cyclodextrin to beta-/ gamma-cyclodextrin
ChainA
Resolution2.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R258 D260 E289 H359 D360
Catalytic site (residue number reindexed from 1) R227 D229 E258 H328 D329
Enzyme Commision number 2.4.1.19: cyclomaltodextrin glucanotransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CA A N170 I221 D230 H264 N139 I190 D199 H233
BS02 CA A D58 D60 N63 N64 G82 D84 D27 D29 N32 N33 G51 D53
Gene Ontology
Molecular Function
GO:0004556 alpha-amylase activity
GO:0016757 glycosyltransferase activity
GO:0030246 carbohydrate binding
GO:0043169 cation binding
GO:0043895 cyclomaltodextrin glucanotransferase activity
GO:0046872 metal ion binding
GO:2001070 starch binding
Biological Process
GO:0005975 carbohydrate metabolic process
Cellular Component
GO:0005576 extracellular region

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3wms, PDBe:3wms, PDBj:3wms
PDBsum3wms
PubMed24637377
UniProtP04830|CDGT1_PAEMA Cyclomaltodextrin glucanotransferase (Gene Name=cgtM)

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